Characterization of an Enterococcus faecium strain in a murine mastitis model.

Autor: Montironi ID; Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina., Moliva MV; Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina., Campra NA; Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina., Raviolo JM; Facultad de Agronomía y Veterinaria, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina., Bagnis G; Facultad de Agronomía y Veterinaria, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina., Cariddi LN; Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina., Reinoso EB; Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto, República Argentina.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2020 May; Vol. 128 (5), pp. 1289-1300. Date of Electronic Publication: 2019 Dec 28.
DOI: 10.1111/jam.14554
Abstrakt: Aim: The aim of the study was to characterize phenotypically and genotypically Enterococcus faecium strains collected from bovine mastitis milk and to evaluate one of them for its virulence in a murine mastitis model.
Methods and Results: A total of five E. faecium isolates were collected from cows with subclinical mastitis. EF-7A showed resistance to antibiotics tested, it presented alpha haemolysin and did not present gelatinase activity. It yielded cyA, efafm and gelE1 genes and it could be characterized as a moderate biofilm producer. It was able to internalize in MAC-T cells and 1×10 8 colony forming unit ml -1 was able to establish an intramammary infection in mice. The strain could be recovered from liver, kidney and blood samples. RAPD profiles showed different bands with respect to the inoculated strain. Histopathology analyses showed different grades of polymorphonuclear neutrophils infiltration in mammary glands.
Conclusion: This is the first report that studied E. faecium strain in a lactating mouse model of mastitis and showed that the experimental inoculation was able to stimulate an inflammatory response resulting in mastitis. Results contribute to a better understanding of intramammary infections caused by E. faecium.
Significance and Impact of the Study: This investigation shows that mice represent a valuable model for the study of the mastitis pathogenesis caused by E. faecium considering the high costs of using cows for mastitis research.
(© 2019 The Society for Applied Microbiology.)
Databáze: MEDLINE
Externí odkaz: