Characterization of the colostrum and transition milk proteomes from primiparous and multiparous Holstein dairy cows.

Autor:	Fahey MJ; Department of Animal and Veterinary Sciences, University of Vermont, Burlington 05405., Fischer AJ; Department of Animal Biosciences, University of Guelph, ON, Canada, N1G 2W1., Steele MA; Department of Animal Biosciences, University of Guelph, ON, Canada, N1G 2W1; Department of Agriculture, Food and Nutritional Science, Edmonton, University of Alberta, Canada, T6G 2P5. Electronic address: masteele@uoguelph.ca., Greenwood SL; Department of Animal and Veterinary Sciences, University of Vermont, Burlington 05405. Electronic address: sabrina.greenwood@uvm.edu.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2020 Feb; Vol. 103 (2), pp. 1993-2005. Date of Electronic Publication: 2019 Dec 16.
DOI:	10.3168/jds.2019-17094
Abstrakt:	Colostrum plays a vital role in the nutrition, development, and immunity of a newborn calf. This study aimed to characterize the protein profile of colostrum and to identify changes in the colostrum proteome across parity during the transition to mature milk. Colostrum and transition milk samples were collected at milkings 1, 2, 4, and 14 after calving from multiparous (n = 10) and primiparous cows (n = 10). Samples were skimmed, fractionated, and enriched before analysis for low-abundance proteins by liquid chromatography-tandem mass spectroscopy (LC-MS/MS). Changes in protein abundances were analyzed using PROC MIXED in SAS (SAS Institute Inc., Cary, NC) with determination of the adaptive false discovery rate adjustment using a MULTTEST procedure to identify effects of parity (P), milking number (MN), and their interaction (MN×P). We identified 86 proteins through LC-MS/MS, including 3 low-abundance proteins that were affected by P, 78 that were affected by MN, and 36 affected by MN×P. Prominent ontological groupings of proteins affected by MN included defense or immunity proteins, such as immunoglobulins. Proteins involved in the plasminogen activating cascade and more broadly, blood coagulation, were affected by MN×P. The results of this study add to increasing knowledge of the colostrum and transition milk proteomes, and this is the first study to find evidence of different abundances of these proteins when examined across P, MN, and MN×P. These findings aid in the identification of potential milk protein biomarkers for mammary health during the early postpartum period. (The Authors. Published by FASS Inc. and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).)
Databáze:	MEDLINE
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