Characterization of triacontyl (C-30) liquid chromatographic columns.

Autor: Sander LC; National Institute of Standards and Technology (NIST), Chemical Sciences Division, 100 Bureau Drive, MS 8392, Gaithersburg, MD 20899-8392, USA. Electronic address: lane.sander@nist.gov., Rimmer CA; National Institute of Standards and Technology (NIST), Chemical Sciences Division, 100 Bureau Drive, MS 8392, Gaithersburg, MD 20899-8392, USA., Wilson WB; National Institute of Standards and Technology (NIST), Chemical Sciences Division, 100 Bureau Drive, MS 8392, Gaithersburg, MD 20899-8392, USA.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2020 Mar 15; Vol. 1614, pp. 460732. Date of Electronic Publication: 2019 Nov 26.
DOI: 10.1016/j.chroma.2019.460732
Abstrakt: Differences in the characteristics of seventeen commercial C-30 liquid chromatographic columns were studied for the separation of carotenoid isomers. A mixture consisting of nine xanthophyll and hydrocarbon carotenoids were separated under conditions carefully chosen to reveal changes in selectivity. The influence of the mobile phase composition, column temperature, and mobile phase flow rate were evaluated. Shape selectivity was characterized with Standard Reference Material (SRM) 869b Column Selectivity Test Mixture, for correlation with carotenoid retention behavior. Regular changes were observed across a broad spectrum of shape selectivity characteristics as indicated by SRM 869b. Better separations of carotenoid isomers were achieved with C-30 columns than were possible with C-18 columns, even after optimization of separation conditions.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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