Expression and activity of AIM2-inflammasome in rheumatoid arthritis patients.

Autor: Méndez-Frausto G; Unidad de Investigación Biomédica. Delegación Zacatecas. Instituto Mexicano del Seguro Social, IMSS, C.P. 98000, Mexico., Medina-Rosales MN; Unidad de Investigación Biomédica. Delegación Zacatecas. Instituto Mexicano del Seguro Social, IMSS, C.P. 98000, Mexico., Uresti-Rivera EE; Centro de Investigación en Ciencias de la Salud y Biomedicina. CICSaB Universidad Autónoma de San Luis Potosí, UASLP, C.P. 78000, Mexico., Baranda-Cándido L; Centro de Investigación en Ciencias de la Salud y Biomedicina. CICSaB Universidad Autónoma de San Luis Potosí, UASLP, C.P. 78000, Mexico; Unidad Regional de Reumatología y Osteoporosis Hospital Central Dr. Ignacio Morones Prieto. San Luis Potosí, SLP, C.P. 78290, Mexico., Zapata-Zúñiga M; Facultad de Medicina y Ciencias de la Salud, Universidad Autónoma de Zacatecas, Hospital Rural No. 51 IMSS Bienestar, Villanueva, Zacatecas, C.P. 99559, Mexico., Bastián Y; Unidad de Investigación Biomédica. Delegación Zacatecas. Instituto Mexicano del Seguro Social, IMSS, C.P. 98000, Mexico; Cátedras CONACYT- Unidad de Investigación Biomédica de Zacatecas-IMSS, Zacatecas, C.P. 98000, Mexico., González Amaro R; Centro de Investigación en Ciencias de la Salud y Biomedicina. CICSaB Universidad Autónoma de San Luis Potosí, UASLP, C.P. 78000, Mexico., Enciso-Moreno JA; Unidad de Investigación Biomédica. Delegación Zacatecas. Instituto Mexicano del Seguro Social, IMSS, C.P. 98000, Mexico., García-Hernández MH; Unidad de Investigación Biomédica. Delegación Zacatecas. Instituto Mexicano del Seguro Social, IMSS, C.P. 98000, Mexico. Electronic address: mariana.garciah@imss.gob.mx.
Jazyk: angličtina
Zdroj: Immunobiology [Immunobiology] 2020 Mar; Vol. 225 (2), pp. 151880. Date of Electronic Publication: 2019 Dec 02.
DOI: 10.1016/j.imbio.2019.11.015
Abstrakt: Introduction: AIM2 inflammasome activation leads to the release of IL-β, which plays an important role in rheumatoid arthritis pathogenesis. In this work, we evaluated AIM2 expression and activity in RA patients and healthy controls.
Methods: AIM2 and RANKL expression were evaluated by flow cytometry. Inflammasome activity was determined in monocyte cultures stimulated with synthetic DNA by measuring IL-1β levels in supernatants using an ELISA assay. The caspase-1 expression in monocytes was measured by western blot, the POP3 expression was analysed by qPCR, and serum levels of IFN-γ were evaluated using ELISA assay.
Results: We observed a diminution of CD14+AIM2+ cells in RA patients, associated with disease activity and evolution. Likewise, the levels of IL-1β were increased in monocyte cultures un-stimulated and stimulated with LPS from RA patients with DAS28 ≥ 4. The Caspase-1 activity and RANKL + monocytes in RA patients were slightly increased. Finally, augmented POP3 expression and diminished IFN-γ serum levels were detected in RA patients.
Conclusion: Our results showed that the monocytes from RA patients were prone to release IL-1β in the absence of the AIM2 inflammasome signal. The down-regulation of AIM2 to a systemic level in RA patients might be a consequence of augmented POP3 expression and might imply the survival of pro-inflammatory cells contributing to the inflammation process.
Competing Interests: Declaration of Competing Interest None of the authors has any potential financial conflict of interest related to this manuscript.
(Copyright © 2019 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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