Lippia sidoides and Lippia origanoides essential oils affect the viability, motility and ultrastructure of Trypanosoma cruzi.

Autor: de Melo ARB; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães, FIOCRUZ, PE, Av. Prof. Moraes Rego s/n Cidade Universitária-Campus da UFPE, Recife, PE, 50670-420, Brazil., Maciel Higino TM; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães, FIOCRUZ, PE, Av. Prof. Moraes Rego s/n Cidade Universitária-Campus da UFPE, Recife, PE, 50670-420, Brazil., da Rocha Oliveira ADP; Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Av. Professor Moraes Rego s/n Cidade Universitária, 50670-901, Recife, PE, Brazil., Fontes A; Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Av. Professor Moraes Rego s/n Cidade Universitária, 50670-901, Recife, PE, Brazil., da Silva DCN; Colegiado de Ciências Biológicas, UNIVASF, Av. José de Sá Maniçoba, S/N CEP 56304-917, Petrolina, PE, Brazil., de Castro MCAB; Núcleo de Enfermagem, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, Brazil., Dantas Lopes JA; Departamento de Química, Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portela-Ininga, Teresina, PI, 64049-550, Brazil., de Figueiredo RCBQ; Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhães, FIOCRUZ, PE, Av. Prof. Moraes Rego s/n Cidade Universitária-Campus da UFPE, Recife, PE, 50670-420, Brazil. Electronic address: bressan@cpqam.fiocruz.br.
Jazyk: angličtina
Zdroj: Micron (Oxford, England : 1993) [Micron] 2020 Feb; Vol. 129, pp. 102781. Date of Electronic Publication: 2019 Nov 12.
DOI: 10.1016/j.micron.2019.102781
Abstrakt: Chagas disease, caused by the protozoan Trypanosoma cruzi, is considered a public health problem. The current chemotherapy for this illness causes serious side effects and its use in the chronic phase of the disease is still controversial. In this regard, the investigation of novel therapeutic strategies remains a priority. The essential oils (EOs) from aromatic plants emerge as a promising source of bioactive compounds. In a previous work we reported the trypanocidal activity of the essential oils from the medicinal plants Lippia sidoides (LSEO) and Lippia origanoides (LOEO) against T. cruzi. Herein, we aimed to further investigate, in more details, the mode of action of LSEO and LOEO on the different developmental stages of this parasite. We showed that Lippia sidoides (LSEO) and Lippia origanoides (LOEO) induced a significant reduction in the percentage of macrophages infected by T. cruzi and in the number of intracellular parasites. Ultrastructural analysis showed that the treatment with both oils caused morphological changes consistent with loss of viability and cell death. The reduced staining with calcein and the increase in the proportion of HE-positive cells also demonstrated that LSEO and LOEO caused loss of parasite viability and membrane integrity. A considerable decrease in Rhodamine 123 and an increase in fluorescence intensity of MitoSox in LOEO were indicative of loss of mitochondrial potential and generation of reactive oxygen species, which ultimately lead to parasite death. Moreover, the optical tweezer analysis indicated that LOEO was more effective in reducing the motility of the epimastigotes. Taken together, our results demonstrated that the LSEO and LOEO are active against T. cruzi and constitute a promising drugs for the therapy of Chagas disease.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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