Mutations in the nucleotide-binding domain of putative sterol importers Aus1 and Pdr11 selectively affect utilization of exogenous sterol species in yeast.

Autor: Papay M; Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences of the Slovak Academy of Sciences, Bratislava, Slovakia., Klein C; Center for Medical Biochemistry, Max Perutz Labs Vienna, Campus Vienna Biocenter, Medical University of Vienna, Vienna, Austria., Hapala I; Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences of the Slovak Academy of Sciences, Bratislava, Slovakia., Petriskova L; Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences of the Slovak Academy of Sciences, Bratislava, Slovakia., Kuchler K; Center for Medical Biochemistry, Max Perutz Labs Vienna, Campus Vienna Biocenter, Medical University of Vienna, Vienna, Austria., Valachovic M; Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences of the Slovak Academy of Sciences, Bratislava, Slovakia.
Jazyk: angličtina
Zdroj: Yeast (Chichester, England) [Yeast] 2020 Jan; Vol. 37 (1), pp. 5-14. Date of Electronic Publication: 2019 Dec 23.
DOI: 10.1002/yea.3456
Abstrakt: Sterol uptake in the yeast Saccharomyces cerevisiae is mediated by two plasma membrane ATP-binding cassette transporters, Aus1 and Pdr11. Their expression is regulated by oxygen and is triggered by anaerobic growth conditions. Under these conditions, internal ergosterol synthesis is arrested and utilization of exogenous sterol is vital for yeast cells. Here, we demonstrate that Aus1 is the major importer of non-yeast sterols, mammalian cholesterol, and plant sterols under anaerobic conditions. In contrast, uptake of yeast native sterol, ergosterol, is relatively low. This uptake could not be enhanced by overexpression of either of the transporters. Interestingly, overexpression of the minor importer Pdr11 resulted in a substantial import of non-yeast sterols. We show that mutation of the conserved residue in one of the ABC characteristic motifs-the H-loop in Aus1 and Pdr11-lowered their ATPase activity. The residual activity was sufficient to import exogenous sterols and to preserve cell viability. Importantly, the reduction of sterol import was dramatic for mammalian cholesterol and plant sterols, whereas import of yeast ergosterol was decreased only slightly indicating substrate selectivity of the sterol utilization process.
(© 2019 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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