Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits.

Autor: Bera SK; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Kamdar JH; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Kasundra SV; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Patel SV; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Jasani MD; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Maurya AK; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Dash P; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Chandrashekar AB; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Rani K; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Manivannan N; National Pulses Research Center, Tamil Nadu Agricultural University (TNAU), Vamban Colony, Pudukkottai, Tamil Nadu, India., Janila P; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India., Pandey MK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India., Vasanthi RP; Regional Agricultural Research Station, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, India., Dobariya KL; Main Oilseeds Research Station, Junagadh Agricultural University (JAU), Junagadh, India., Radhakrishnan T; Indian Council of Agricultural Research-Directorate of Groundnut Research (ICAR-DGR), Junagadh, India., Varshney RK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Dec 12; Vol. 14 (12), pp. e0226252. Date of Electronic Publication: 2019 Dec 12 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0226252
Abstrakt: Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust.
Competing Interests: All authors have contributed equally to this research work and have no competing interests exist.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje