Soluble electron acceptors affect bioluminescence from Shewanella woodyi.

Autor: Theberge AL; Chemistry Department, University of Dayton, 300 College Park, Dayton, OH, USA., Alsabia SM; Chemistry Department, University of Dayton, 300 College Park, Dayton, OH, USA., Mortensen CT; Chemistry Department, University of Dayton, 300 College Park, Dayton, OH, USA., Blair AG; Chemistry Department, University of Dayton, 300 College Park, Dayton, OH, USA., Wendel NM; Chemistry Department, University of Dayton, 300 College Park, Dayton, OH, USA., Biffinger JC; Chemistry Department, University of Dayton, 300 College Park, Dayton, OH, USA.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2020 May; Vol. 35 (3), pp. 427-433. Date of Electronic Publication: 2019 Dec 12.
DOI: 10.1002/bio.3744
Abstrakt: Shewanella woodyi cultures were used to correlate bioluminescence intensity with changes in the electrochemical potential of a saltwater medium using soluble electron acceptors. A relationship between the concentration of NaNO 3 or CoCl 2 to bioluminescence intensity was confirmed using aerobic cultures of S. woodyi at 20°C with glucose as the sole carbon source. In general, increasing the concentration of nitrate or Co(II) reduced the bioluminescence per cell, with complete luminescence being repressed at ≥5 mM nitrate and ≥0.5 mM Co(II). Results from cell viability fluorescent staining concluded that increasing the concentration of Co(II) or nitrate did not affect the overall viability of the cells when compared with cultures lacking Co(II) or nitrate. These data show that potentials of <0.2 V vs Normal Hydrogen Electrode (NHE) repress the luminescence from the cells, but the exact mechanism is unclear. Our results indicated that the luminescence intensity from S. woodyi could be systematically reduced using these two soluble electron acceptors, making S. woodyi a potential model bacterium for whole-cell luminescence bioelectrochemical sensor applications.
(© 2019 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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