Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar 'Micro-Tom'.

Autor:	Choi SW; Department of Natural Sciences, International Christian University (ICU), 3-10-2 Osawa, Mitaka, Tokyo 181-8585, Japan., Hoshikawa K; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan., Fujita S; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan., Thi DP; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan., Mizoguchi T; Department of Natural Sciences, International Christian University (ICU), 3-10-2 Osawa, Mitaka, Tokyo 181-8585, Japan., Ezura H; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan., Ito E; Department of Natural Sciences, International Christian University (ICU), 3-10-2 Osawa, Mitaka, Tokyo 181-8585, Japan.
Jazyk:	angličtina
Zdroj:	Plant biotechnology (Tokyo, Japan) [Plant Biotechnol (Tokyo)] 2018 Sep 25; Vol. 35 (3), pp. 225-235.
DOI:	10.5511/plantbiotechnology.18.0525a
Abstrakt:	Quantitative real-time PCR (qRT-PCR) is widely used to analyze the expression profiles of the genes of interest. In order to obtain accurate quantification data, normalization by using reliable internal control genes is essential. In this study, we evaluated the stability and applicability of eight internal control gene candidates for analyzing gene expression during fruit development in dwarf tomato cultivar Micro-Tom. We collected seventeen different samples from flowers and fruits at different developmental stages, and estimated the expression stability of the candidate genes by two statistical algorithms, geNorm and NormFinder. The combined ranking order and qRT-PCR analyses for expression profiles of SlYABBY2a , SlYABBY1a , FRUITFULL1 and APETALA2c suggested that EXPRESSED was the most stable and reliable internal control gene among the candidates. Our analysis also suggested that RPL8 was also suitable if the sample group is limited to fruits at different maturation stages. In addition to EXPRESSED , GAPDH was also applicable for relative quantitation to monitor gene expression profiles through fruit development from pistil to pericarp. (© 2018 The Japanese Society for Plant Cell and Molecular Biology.)
Databáze:	MEDLINE
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