Comparative Study Of Genetic Diversity, Virulence Genotype, Biofilm Formation And Antimicrobial Resistance Of Uropathogenic Escherichia coli (UPEC) Isolated From Nosocomial And Community Acquired Urinary Tract Infections.

Autor: De Souza GM; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil., Neto ERDS; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil., da Silva AM; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil., Iacia MVMS; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil., Rodrigues MVP; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil., Cataneli Pereira V; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil., Winkelstroter LK; Health Sciences Faculty, University of Western Sao Paulo, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: Infection and drug resistance [Infect Drug Resist] 2019 Nov 22; Vol. 12, pp. 3595-3606. Date of Electronic Publication: 2019 Nov 22 (Print Publication: 2019).
DOI: 10.2147/IDR.S228612
Abstrakt: Introduction: Escherichia coli is a Gram-negative opportunistic human pathogen, which has aroused considerable medical interest for being involved in cases of urinary tract infection.
Aim: Characterize the E. coli isolated both in the hospital and in the community.
Methodology: A total of 200 E. coli isolated in urine samples from hospital and community were evaluated in biofilm formation assay and hydrophobicity MATS method. Antimicrobial susceptibility was performed through agar-diffusion technique. Virulence and ESBL production genes were observed through the polymerase chain reaction amplification of papC, fimH, fliC, kpsMTII, bla TEM , bla CTX-M, bla SHV , and bla OXA. The phylogenetic classification was based on the pattern chuA and yjaA and the region TspE4.C2 by PCR Multiplex.
Results: A higher frequency of non-adherent or poorly adherent isolates was observed in the community group. Approximately 85% of the community isolates were distributed in the highest hydrophilicity group (p<0.05). The level of resistant microorganisms was present at the same level in both source (p>0.05). About 14% of the hospital isolates were positive in the ESBL phenotypic detection test (p>0.05). Among the samples, 95% presented ESBL-encoding genes. The predominant phylogenetic group was B2 (78%). Community isolates showed a higher prevalence of virulence genes fimH, papC , and kpsMTII when compared to hospital samples.
Conclusion: These data confirm the worldwide trend that isolates in the community present sometimes higher levels of virulence and antimicrobial resistance.
Competing Interests: The authors declare that they have no conflicts of interest in this work.
(© 2019 De Souza et al.)
Databáze: MEDLINE
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