First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil.

Autor: Rosa UA; Institute of Biological Sciences, Federal University of Para, Para, 66075-000, Brazil., Ribeiro GO; Institute of Biological Sciences, Federal University of Para, Para, 66075-000, Brazil., Villanova F; Institute of Biological Sciences, Federal University of Para, Para, 66075-000, Brazil., Luchs A; Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Sao Paulo, 01246-000, Brazil., Milagres FAP; Secretary of Health of Tocantins, Tocantins, 77453-000, Brazil.; Institute of Biological Sciences, Federal University of Tocantins, Tocantins, 77001-090, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, 77016-330, Brazil., Komninakis SV; Postgraduate Program in Health Science, Faculty of Medicine of ABC, Santo André, 09060-870, Brazil.; Retrovirology Laboratory, Federal University of São Paulo, São Paulo, 04023-062, Brazil., Tahmasebi R; Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, 05403-000, Brazil.; Polytechnic School of University of Sao Paulo, Sao Paulo, Brazil., Lobato MCABS; Secretary of Health of Tocantins, Tocantins, 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, 77016-330, Brazil., Brustulin R; Secretary of Health of Tocantins, Tocantins, 77453-000, Brazil.; Institute of Biological Sciences, Federal University of Tocantins, Tocantins, 77001-090, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, 77016-330, Brazil., Chagas RTD; Secretary of Health of Tocantins, Tocantins, 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, 77016-330, Brazil., Abrão MFNDS; Secretary of Health of Tocantins, Tocantins, 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, 77016-330, Brazil., Soares CVDA; Secretary of Health of Tocantins, Tocantins, 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins, 77016-330, Brazil., Tinker RJ; Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, 05403-000, Brazil.; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PL, UK., Pandey RP; Centre for Drug Design Discovery and Development (C4D), SRM University, Delhi-NCR, Rajiv Gandhi Education City, Sonepat, 131 029, Haryana, India., Raj VS; Centre for Drug Design Discovery and Development (C4D), SRM University, Delhi-NCR, Rajiv Gandhi Education City, Sonepat, 131 029, Haryana, India., Sabino EC; Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, 05403-000, Brazil.; LIM/46, Faculdade de Medicina, Universidade de São Paulo, São Paulo, 01246-903, Brazil., Deng X; Vitalant Research Institute, San Francisco, CA, 94143, USA.; Department Laboratory Medicine, University of California San Francisco, San Francisco, CA, 94143, USA., Delwart E; Vitalant Research Institute, San Francisco, CA, 94143, USA.; Department Laboratory Medicine, University of California San Francisco, San Francisco, CA, 94143, USA., Costa ACD; Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, 05403-000, Brazil. charlysbr@yahoo.com.br., Leal É; Institute of Biological Sciences, Federal University of Para, Para, 66075-000, Brazil. elcioleal@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Dec 09; Vol. 9 (1), pp. 18599. Date of Electronic Publication: 2019 Dec 09.
DOI: 10.1038/s41598-019-55216-5
Abstrakt: Diarrhea remains one of the most common causes of deaths in children. Although many studies have investigated the prevalence of enteric pathogens around the globe some diarrheal episodes remain unexplained. It is possible that some yet-unidentified viral agents could be related to these cases of gastroenteritis. By using viral metagenomics techniques, we screened 251 fecal samples of children between 0.5 to 2.5-year-old with acute diarrhea not associated with common pathogens. These children live in rural areas and have different levels of contact with animals such as pigs, cows and bats. Here we report a complete genome of one mammalian orthoreovirus (MRV) type 3, denoted TO-151/BR, detected in a female child in the state of Tocantins (north of Brazil). Brazilian TO-151/BR strain was classified as MRV-3 based on S1 phylogeny and was closely related to porcine Asian strains. Phylogenetic analyses showed that other segments were more similar to MRV-3s of different geographic locations and hosts, including human and bats, highlighting genome reassortment and lack of host-specific barriers. This is the first report of MRV-3 in South America and a hypothesis of a silent long-term circulation of this virus in Brazil has been raised.
Databáze: MEDLINE
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