MERLIN: a novel BRET-based proximity biosensor for studying mitochondria-ER contact sites.

Autor:	Hertlein V; Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany., Flores-Romero H; Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany., Das KK; Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany., Fischer S; University of Heidelberg, Heidelberg, Germany., Heunemann M; Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany., Calleja-Felipe M; Molecular Cognition Laboratory, Biophysics Institute, Consejo Superior de Investigaciones Cientificas, University of the Basque Country (UPV)/Euskal Herriko University, Campus Universidad del País Vasco, Leioa, Spain., Knafo S; Molecular Cognition Laboratory, Biophysics Institute, Consejo Superior de Investigaciones Cientificas, University of the Basque Country (UPV)/Euskal Herriko University, Campus Universidad del País Vasco, Leioa, Spain.; Ikerbasque, Basque Foundation for Science, Bilbao, Spain.; Department of Physiology and Cell Biology and National Institute of Biotechnology in the Negev, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel., Hipp K; Max Planck Institute for Developmental Biology, Tübingen, Germany., Harter K; Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany., Fitzgerald JC; Hertie-Institute for Clinical Brain Research, University of Tübingen and German Centre for Neurodegenerative Diseases (DZNE), Tübingen, Germany., García-Sáez AJ; Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany ana.garcia@uni-koeln.de.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2019 Dec 09; Vol. 3 (1). Date of Electronic Publication: 2019 Dec 09 (Print Publication: 2020).
DOI:	10.26508/lsa.201900600
Abstrakt:	The contacts between the ER and mitochondria play a key role in cellular functions such as the exchange of lipids and calcium between both organelles, as well as in apoptosis and autophagy signaling. The molecular architecture and spatiotemporal regulation of these distinct contact regions remain obscure and there is a need for new tools that enable tackling these questions. Here, we present a new bioluminescence resonance energy transfer-based biosensor for the quantitative analysis of distances between the ER and mitochondria that we call MERLIN (Mitochondria-ER Length Indicator Nanosensor). The main advantages of MERLIN compared with available alternatives are that it does not rely on the formation of artificial physical links between the two organelles, which could lead to artifacts, and that it allows to study contact site reversibility and dynamics. We show the applicability of MERLIN by characterizing the role of the mitochondrial dynamics machinery on the contacts of this organelle with the ER. (© 2019 Hertlein et al.)
Databáze:	MEDLINE
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