Evaluating viscoelastic properties and membrane electrical charges of red blood cells with optical tweezers and cationic quantum dots - applications to β-thalassemia intermedia hemoglobinopathy.

Autor: Lima CN; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil., Moura DS; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil; Colégio de Aplicação, Universidade Federal de Pernambuco, Recife, PE, Brazil., Silva YSS; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil., Souza TH; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil., Crisafuli FAP; Instituto Federal Farroupilha, Alegrete, RS, Brazil., Silva DCN; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil; Colegiado de Ciências Biológicas, Campus de Ciências Agrárias, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil., Peres JC; Fundação Hemope, Recife, PE, Brazil., Cesar CL; Departamento de Física, Universidade Federal do Ceará, Fortaleza, CE, Brazil; Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, Campinas, SP, Brazil., de Araujo RE; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil., Fontes A; Laboratório de Óptica Biomédica e Imagens, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address: adriana.fontes@ufpe.br.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2020 Feb; Vol. 186, pp. 110671. Date of Electronic Publication: 2019 Nov 25.
DOI: 10.1016/j.colsurfb.2019.110671
Abstrakt: Biomechanical and electrical properties are important to the performance and survival of red blood cells (RBCs) in the microcirculation. This study proposed and explored methodologies based on optical tweezers and cationic quantum dots (QDs) as biophotonic tools to characterize, in a complementary way, viscoelastic properties and membrane electrical charges of RBCs. The methodologies were applied to normal (HbA) and β-thalassemia intermedia (Hbβ) RBCs. The β-thalassemia intermedia disease is a hereditary hemoglobinopathy characterized by a reduction (or absence) of β-globin chains, which leads to α-globin chains precipitation. The apparent elasticity (μ) and membrane viscosity (η m ) of RBCs captured by optical tweezers were obtained in just a single experiment. Besides, the membrane electrical charges were evaluated by flow cytometry, exploring electrostatic interactions between cationic QDs, stabilized with cysteamine, with the negatively charged RBC surfaces. Results showed that Hbβ RBCs are less elastic, have a higher η m , and presented a reduction in membrane electrical charges, when compared to HbA RBCs. Moreover, the methodologies based on optical tweezers and QDs, here proposed, showed to be capable of providing a deeper and integrated comprehension on RBC rheological and electrical changes, resulting from diverse biological conditions, such as the β-thalassemia intermedia hemoglobinopathy.
Competing Interests: Declaration of Competing Interest The authors declare no competing or financial interests.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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