Electrospun nanofibers - A promising solid in-situ gelling alternative for ocular drug delivery.

Autor: Göttel B; Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120 Halle (Saale), Germany., de Souza E Silva JM; Institute of Physics, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Str. 4, 06120 Halle (Saale), Germany., Santos de Oliveira C; Institute of Physics, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Str. 4, 06120 Halle (Saale), Germany., Syrowatka F; The Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Str. 4, 06120 Halle (Saale), Germany., Fiorentzis M; Department of Ophthalmology, Martin Luther University Halle-Wittenberg, University Medicine Halle, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany., Viestenz A; Department of Ophthalmology, Martin Luther University Halle-Wittenberg, University Medicine Halle, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany., Viestenz A; Department of Ophthalmology, Martin Luther University Halle-Wittenberg, University Medicine Halle, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany., Mäder K; Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120 Halle (Saale), Germany. Electronic address: karsten.maeder@pharmazie.uni-halle.de.
Jazyk: angličtina
Zdroj: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2020 Jan; Vol. 146, pp. 125-132. Date of Electronic Publication: 2019 Dec 07.
DOI: 10.1016/j.ejpb.2019.11.012
Abstrakt: A serious problem of the treatment of eye diseases is the very short residence time of the drug. The majority of the drug is cleared within few seconds due to the poor capability of the eye to accommodate additional liquids. We developed a new ocular drug delivery system, which is applied in dry form and forms immediately a gel after administration. The system is based on gellan gum/pullulan electrospun nanofibers. The rheological behavior of the spinning solution was investigated followed by further characterization of the in situ formed gel. Three-dimensional X-ray imaging with nanometric resolution (nano-CT) and electron scanning microscopy were used for a detailed characterization of the diameter and alignment of the fibers. A high porosity (87.5 ± 0.5%) and pore interconnectivity (99%) was found. To ensure a good fit to the eye anatomy, the prepared fibers were shaped into curved geometries. Additionally, a new innovative moistening chamber for the in vitro determination of the ocular residence time in porcine eyes was developed which mimics the tear turnover. A clear prolongation of the fluorescein residence time compared to conventional eye drops was achieved with the application of the curved nanofiber in situ gelling mat. In summary, the developed in situ gelling system with adapted geometry is a promising alternative system for ocular drug delivery.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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