FORSCells: 40-days fixed prepared reagent for detection of anti-Forssman in humans.

Autor: Ferreira S; Polytechnic of Coimbra, ESTeSC, Departament of Biomedical Laboratory Sciences, ESTeSC, Rua 5 de Outubro, SM Bispo, Apartado 7006, 3046-854, Coimbra, Portugal., Mourato C; Polytechnic of Coimbra, ESTeSC, Departament of Biomedical Laboratory Sciences, ESTeSC, Rua 5 de Outubro, SM Bispo, Apartado 7006, 3046-854, Coimbra, Portugal., Corpuz A; School of Biological Sciences, Dublin Institute of Technology, Kevin St, Dublin 8, Ireland., Galvão S; Polytechnic of Coimbra, ESTeSC, Departament of Biomedical Laboratory Sciences, ESTeSC, Rua 5 de Outubro, SM Bispo, Apartado 7006, 3046-854, Coimbra, Portugal., Hesse C; Institutionen för biomedicin, Sahlgrenska Akademin vid, Göteborgs Universitet, Sweden., Rocha C; Polytechnic of Coimbra, ESTeSC, Department Complementary Sciences, ESTeSC, Rua 5 de Outubro, SM Bispo, Apartado 7006, 3046-854, Coimbra, Portugal; Institute for Systems Engineering and Computers at Coimbra, Coimbra, Portugal., Jesus C; Polytechnic of Coimbra, ESTeSC, Departament of Biomedical Laboratory Sciences, ESTeSC, Rua 5 de Outubro, SM Bispo, Apartado 7006, 3046-854, Coimbra, Portugal., Mendes F; Polytechnic of Coimbra, ESTeSC, Departament of Biomedical Laboratory Sciences, ESTeSC, Rua 5 de Outubro, SM Bispo, Apartado 7006, 3046-854, Coimbra, Portugal; Biophysics Institute, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal; CNC.IBILI Consortium/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Azinhaga de Santa Comba, Celas, 3000-548 Coimbra, Portugal. Electronic address: fjmendes@estescoimbra.pt.
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 2020 Mar; Vol. 478, pp. 112722. Date of Electronic Publication: 2019 Dec 06.
DOI: 10.1016/j.jim.2019.112722
Abstrakt: In 2012, the FORS system was accepted by the International Society of Blood Transfusion as the 31 st blood group system. Forssman (Fs) antigen (Ag) expression is most commonly found on sheep red blood cells (RBC) but rare in human RBC. Anti-Fs antibodies (Ab) are naturally occurring in human sera and are predominantly IgM but they can also be IgG. To this day, the global prevalence of the FORS system is unknown. Currently, there is a lack of natural FORS1-positive RBC available to use for anti-Fs screening in large populations. This study was designed to produce FORS1-positive cells viable for 40 days use in the anti-Fs screening. Three to 5% FORS1-positive cells were produced using sheep's blood and CellStab stabilizer solution. The quality of the FORS1-positive cells was investigated in more than three independent experiments of ABO titration, osmotic fragility test and supernatant haemolysis. For each batch of FORS1-positive cells produced, an extended antibody panel was performed. To demonstrate that the FORS1-positive cells can be used for up to 40 days, anti-Fs screening and classification were carried out in a patient and donor population. Antigenic expression and membrane integrity of FORS1-positive cells remained stable for 40 days. Good FORS1 Ag preservation was established, and minimal haemolysis was observed. In conclusion, a novel and easy-to-produce reagent has been developed and submitted to a patent with stable FORS1 Ag expression. With this FORS1-positive cell suspension, it is now possible to screen and classify anti-Fs Ab in large populations.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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