Next-generation sequencing of microbial cell-free DNA for rapid noninvasive diagnosis of infectious diseases in immunocompromised hosts.
| Autor: | Camargo JF; Infectious Diseases, University of Miami Miller School of Medicine, Miami, FL, 33136, USA., Ahmed AA; Karius, Inc., Redwood City, CA, USA., Lindner MS; Karius, Inc., Redwood City, CA, USA., Morris MI; Infectious Diseases, University of Miami Miller School of Medicine, Miami, FL, 33136, USA., Anjan S; Infectious Diseases, University of Miami Miller School of Medicine, Miami, FL, 33136, USA., Anderson AD; Department of Pharmacy, University of Miami Sylvester Comprehensive Cancer Center, Miami, FL, 33136, USA., Prado CE; Department of Microbiology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA., Dalai SC; Karius, Inc., Redwood City, CA, USA., Martinez OV; Department of Microbiology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA., Komanduri KV; Division of Hematology Oncology, University of Miami Miller School of Medicine, Miami, FL, 33136, USA. |
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| Jazyk: | angličtina |
| Zdroj: | F1000Research [F1000Res] 2019 Jul 26; Vol. 8, pp. 1194. Date of Electronic Publication: 2019 Jul 26 (Print Publication: 2019). |
| DOI: | 10.12688/f1000research.19766.2 |
| Abstrakt: | Background: Cell-free DNA (cfDNA) sequencing has emerged as an effective laboratory method for rapid and noninvasive diagnosis in prenatal screening testing, organ transplant rejection screening, and oncology liquid biopsies. Methods: Here we report our experience using next-generation sequencing (NGS) for detection of microbial cfDNA in a cohort of ten immunocompromised patients with febrile neutropenia, pneumonia or intra-abdominal infection. Results: Among five hematological malignancy patients, for whom a microbiological diagnosis was established, pathogen identification by cfDNA NGS demonstrated 100% positive agreement with conventional diagnostic laboratory methods. Further, cfDNA identified the etiological agent in two patients with culture negative sepsis who had undergone hematopoietic stem cell transplant. Conclusion: These data support the clinical utility of measurement of microbial cfDNA sequencing from peripheral blood for rapid noninvasive diagnosis of infections in immunocompromised hosts. Larger studies are needed. Competing Interests: Competing interests: Karius, Inc. ran the tests on the clinical specimens for these 10 patients at no charge to our institution. (Copyright: © 2019 Camargo JF et al.) |
| Databáze: | MEDLINE |
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