Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy.

Autor: Ortega-Martínez M; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Monterrey, Nuevo León, 64460, Mexico., Gutiérrez-Dávila V; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Monterrey, Nuevo León, 64460, Mexico., Niderhauser-García A; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Monterrey, Nuevo León, 64460, Mexico., Salazar-Aranda R; Department of Analytical Chemistry, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Monterrey, Nuevo León, 64460, Mexico., Solís-Soto JM; Department of Physiology, School of Dentistry, Autonomous University of Nuevo Leon, Dr. Eduardo Aguirre P. y Silao, Monterrey, Nuevo León, 64460, Mexico., Montes-de-Oca-Luna R; Department of Histology, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Monterrey, Nuevo León, 64460, Mexico., Jaramillo-Rangel G; Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Monterrey, Nuevo León, 64460, Mexico.
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2020 Mar; Vol. 44 (3), pp. 918-923. Date of Electronic Publication: 2020 Jan 21.
DOI: 10.1002/cbin.11280
Abstrakt: Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: macropexophagy and micropexophagy. Previous studies showed that peroxisomes damaged by a prolonged exposition to PA1 are eliminated by macropexophagy. In this work, Candida boidinii was grown in methanol-containing media, and PA1 was added to the cultures at 2 µg/mL after they reached the mid-exponential growth phase. Samples were taken at 5, 10, 15, 20, and 25 min after the addition of PA1 and processed for ultrastructural analysis. Typical morphological characteristics of micropexophagy were observed: the direct engulfment of peroxisomes by the vacuolar membrane and the presence of the micropexophagic membrane apparatus (MIPA), which mediates the fusion between the opposing tips of the vacuole to complete sequestration of peroxisomes from the cytosol. In conclusion, here we report that, in addition to macropexophagy, peroxisomes damaged by PA1 can be eliminated by micropexophagy. This information is useful to deepen the knowledge of the mechanism of action of PA1 and of that of pexophagy per se.
(© 2019 International Federation for Cell Biology.)
Databáze: MEDLINE
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