Application of the fentanyl analog screening kit toward the identification of emerging synthetic opioids in human plasma and urine by LC-QTOF.

Autor: Krajewski LC; Battelle Memorial Institute at the Centers for Disease Control and Prevention, Atlanta, GA, 30341, USA., Swanson KD; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA., Bragg WA; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA., Shaner RL; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA. Electronic address: rebecca.shaner@cdc.hhs.gov., Seymour C; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA., Carter MD; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA., Hamelin EI; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA., Johnson RC; Division of Laboratory Sciences, National Center for Environmental Health, CDC, Atlanta, GA, 30341, USA.
Jazyk: angličtina
Zdroj: Toxicology letters [Toxicol Lett] 2020 Mar 01; Vol. 320, pp. 87-94. Date of Electronic Publication: 2019 Dec 05.
DOI: 10.1016/j.toxlet.2019.12.007
Abstrakt: Human exposures to fentanyl analogs, which significantly contribute to the ongoing U.S. opioid overdose epidemic, can be confirmed through the analysis of clinical samples. Our laboratory has developed and evaluated a qualitative approach coupling liquid chromatography and quadrupole time-of-flight mass spectrometry (LC-QTOF) to address novel fentanyl analogs and related compounds using untargeted, data-dependent acquisition. Compound identification was accomplished by searching against a locally-established mass spectral library of 174 fentanyl analogs and metabolites. Currently, our library can identify 150 fentanyl-related compounds from the Fentanyl Analog Screening (FAS) Kit), plus an additional 25 fentanyl-related compounds from individual purchases. Plasma and urine samples fortified with fentanyl-related compounds were assessed to confirm the capabilities and intended use of this LC-QTOF method. For fentanyl, 8 fentanyl-related compounds and naloxone, lower reportable limits (LRL 100 ), defined as the lowest concentration with 100 % true positive rate (n = 12) within clinical samples, were evaluated and range from 0.5 ng/mL to 5.0 ng/mL for urine and 0.25 ng/mL to 2.5 ng/mL in plasma. The application of this high resolution mass spectrometry (HRMS) method enables the real-time detection of known and emerging synthetic opioids present in clinical samples.
(Published by Elsevier B.V.)
Databáze: MEDLINE
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