Detection of clinically relevant epidermal growth factor receptor pathway mutations in circulating cell-free tumor DNA using next generation sequencing in squamous cell carcinoma lung.

Autor:	Govind KB; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Koppaka D; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Dasappa L; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Jacob LA; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., C Babu SM; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Lokesh NK; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Haleshappa RA; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Rajeev LK; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Saldanha SC; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Abhishek A; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Asati V; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Chethan R; Department of Medical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India., Ramprasad VL; Medgenome Labs, Bommasandra, Bangalore, Karnataka, India.
Jazyk:	angličtina
Zdroj:	South Asian journal of cancer [South Asian J Cancer] 2019 Oct-Dec; Vol. 8 (4), pp. 247-249.
DOI:	10.4103/sajc.sajc_281_18
Abstrakt:	Background: Limited repertoires of targets are available in the management of squamous cell carcinoma lung. In this study, we analyzed epidermal growth factor receptor (EGFR), RAS, BRAF mutations in lung cancer patients of squamous cell histology using next-generation sequencing (NGS) on the circulating cell-free DNA (cf-DNA). Materials and Methods: In this prospective observational study, patients with squamous cell carcinoma lung, either newly diagnosed or having a progressive disease on prior therapy were eligible. Cf-DNA was extracted from peripheral blood and analyzed for EGFR, KRAS, NRAS, and BRAF mutations using NGS. Results: Sixteen patients were enrolled over a period of 1 month. The mean cf-DNA quantity extracted from the plasma was 96.5 ng (range, 15-200 ng). Eight clinically relevant mutations in the EGFR pathway were identified. These include Exon 21 mutations in 4 patients, Exon 20 mutation in onepatient, complex mutations with coexisting Exon 21 and Exon18 in one patient and KRAS Exon 2 mutations in two patients. Conclusion: cf-DNA is a minimally invasive technique for detection of clinically relevant mutations in lung cancer patients. The use of novel advanced techniques such as NGS may help in detecting EGFR pathway mutations in patients with squamous cell carcinoma lung. Competing Interests: There are no conflicts of interest. (Copyright: © 2019 The South Asian Journal of Cancer.)
Databáze:	MEDLINE
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