Aurora B functions at the apical surface after specialized cytokinesis during morphogenesis in C. elegans .

Autor:	Bai X; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Melesse M; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Sorensen Turpin CG; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Sloan DE; Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Chen CY; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan., Wang WC; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan., Lee PY; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan., Simmons JR; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Nebenfuehr B; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Mitchell D; Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA., Klebanow LR; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Mattson N; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA., Betzig E; Janelia Research Campus, HHMI, Ashburn, VA 20147, USA., Chen BC; Janelia Research Campus, HHMI, Ashburn, VA 20147, USA.; Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan., Cheerambathur D; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UK., Bembenek JN; Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA bembenek@umich.edu.; Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2020 Jan 08; Vol. 147 (1). Date of Electronic Publication: 2020 Jan 08.
DOI:	10.1242/dev.181099
Abstrakt:	Although cytokinesis has been intensely studied, the way it is executed during development is not well understood, despite a long-standing appreciation that various aspects of cytokinesis vary across cell and tissue types. To address this, we investigated cytokinesis during the invariant Caenorhabditis elegans embryonic divisions and found several parameters that are altered at different stages in a reproducible manner. During early divisions, furrow ingression asymmetry and midbody inheritance is consistent, suggesting specific regulation of these events. During morphogenesis, we found several unexpected alterations to cytokinesis, including apical midbody migration in polarizing epithelial cells of the gut, pharynx and sensory neurons. Aurora B kinase, which is essential for several aspects of cytokinesis, remains apically localized in each of these tissues after internalization of midbody ring components. Aurora B inactivation disrupts cytokinesis and causes defects in apical structures, even if inactivated post-mitotically. Therefore, we demonstrate that cytokinesis is implemented in a specialized way during epithelial polarization and that Aurora B has a role in the formation of the apical surface. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2020. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu