Spatial EGFR Dynamics and Metastatic Phenotypes Modulated by Upregulated EphB2 and Src Pathways in Advanced Prostate Cancer.

Autor: Liu YL; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA., Horning AM; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Lieberman B; Department of Biology, Trinity University, San Antonio, TX 78212, USA., Kim M; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA., Lin CK; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Hung CN; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Chou CW; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Wang CM; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Lin CL; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Kirma NB; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Liss MA; Department of Urology, University of Texas Health Science Center, San Antonio, TX 78229, USA., Vasisht R; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA., Perillo EP; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA., Blocher K; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA., Horng H; Department of Bioengineering, the University of Maryland, College Park, MD 20742, USA., Taverna JA; Department of Medicine, Mays Cancer Center, University of Texas Health Science Center, San Antonio, TX 78229, USA., Ruan J; Department of Computer Science, University of Texas at San Antonio, TX 78249, USA., Yankeelov TE; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA.; Institute for Computational Engineering and Sciences, University of Texas at Austin, Austin, TX 78712, USA.; Department of Diagnostic Medicine, Dell Medical School, University of Texas at Austin, Austin, TX 78712, USA.; Department of Oncology, Dell Medical School, University of Texas at Austin, Austin, TX 78712, USA.; Livestrong Cancer Institutes, University of Texas at Austin, Austin, TX 78712, USA., Dunn AK; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA., Huang TH; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA., Yeh HC; Department of Biomedical Engineering, University of Texas at Austin, 107 W. Dean Keeton, BME Building, Austin, TX 78712, USA.; Texas Materials Institute, University of Texas at Austin, Austin, TX 78712, USA., Chen CL; Department of Molecular Medicine, Mays Cancer Center, University of Texas Health Science Center, 8210 Floyd Curl Drive, Mail code: 8257 , San Antonio, TX 78229, USA.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2019 Dec 01; Vol. 11 (12). Date of Electronic Publication: 2019 Dec 01.
DOI: 10.3390/cancers11121910
Abstrakt: Advanced prostate cancer is a very heterogeneous disease reflecting in diverse regulations of oncogenic signaling pathways. Aberrant spatial dynamics of epidermal growth factor receptor (EGFR) promote their dimerization and clustering, leading to constitutive activation in oncogenesis. The EphB2 and Src signaling pathways are associated with the reorganization of the cytoskeleton leading to malignancy, but their roles in regulating EGFR dynamics and activation are scarcely reported. Using single-particle tracking techniques, we found that highly phosphorylated EGFR in the advanced prostate cancer cell line, PC3, was associated with higher EGFR diffusivity, as compared with LNCaP and less aggressive DU145. The increased EGFR activation and biophysical dynamics were consistent with high proliferation, migration, and invasion. After performing single-cell RNA-seq on prostate cancer cell lines and circulating tumor cells from patients, we identified that upregulated gene expression in the EphB2 and Src pathways are associated with advanced malignancy. After dasatinib treatment or siRNA knockdowns of EphB2 or Src, the PC3 cells exhibited significantly lower EGFR dynamics, cell motility, and invasion. Partial inhibitory effects were also found in DU145 cells. The upregulation of parts of the EphB2 and Src pathways also predicts poor prognosis in the prostate cancer patient cohort of The Cancer Genome Atlas. Our results provide evidence that overexpression of the EphB2 and Src signaling pathways regulate EGFR dynamics and cellular aggressiveness in some advanced prostate cancer cells.
Competing Interests: The authors declare that they have no competing interests.
Databáze: MEDLINE
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