RADIATION-INDUCED PLASMID DNA DAMAGE: EFFECT OF CONCENTRATION AND LENGTH.
Autor: | Brabcová KP; Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS, Na Truhlářce 39/64, 180 00 Praha, Czech Republic., Jamborová Z; Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 78/7, 110 00 Praha, Czech Republic., Michaelidesová A; Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS, Na Truhlářce 39/64, 180 00 Praha, Czech Republic.; Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 78/7, 110 00 Praha, Czech Republic., Davídková M; Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS, Na Truhlářce 39/64, 180 00 Praha, Czech Republic., Kodaira S; National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage, 263-8555 Chiba, Japan., Šefl M; Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS, Na Truhlářce 39/64, 180 00 Praha, Czech Republic.; Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Břehová 78/7, 110 00 Praha, Czech Republic., Štěpán V; Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS, Na Truhlářce 39/64, 180 00 Praha, Czech Republic. |
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Jazyk: | angličtina |
Zdroj: | Radiation protection dosimetry [Radiat Prot Dosimetry] 2019 Dec 31; Vol. 186 (2-3), pp. 168-171. |
DOI: | 10.1093/rpd/ncz196 |
Abstrakt: | Plasmid DNA is commonly used as a simpler substitute for a cell in studies of early effects of ionizing radiation because it allows to determine yields of primary DNA lesions. Experimental studies often employ plasmids of different lengths, in different concentrations in the aqueous solution. Influence of these parameters on the heavy-ion induced yields of primary DNA damage has been studied, using plasmids pUC19 (2686 bp), pBR322 (4361 bp) and pKLAC2 (9107 bp) in 10 and 50 ng/μl concentration. Results demonstrate the impact of plasmid length, while no significant difference was observed between the two concentrations. The uncertainty of the results is discussed. (© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.) |
Databáze: | MEDLINE |
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