Expression and characterization of the non-structural protein V of small ruminant morbillivirus.

Autor: Yadav AK; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Chaudhary D; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Bhadouriya S; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Chandrasekar S; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Dhanesh VV; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Rajak KK; 2ICAR, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India., Singh RP; 2ICAR, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India., Ramakrishnan MA; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Singh RK; 2ICAR, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India., Muthuchelvan D; 1ICAR, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India.
Jazyk: angličtina
Zdroj: Virusdisease [Virusdisease] 2019 Sep; Vol. 30 (3), pp. 465-468. Date of Electronic Publication: 2019 Jul 24.
DOI: 10.1007/s13337-019-00539-0
Abstrakt: Peste-des-petits ruminants is a transboundary viral disease of small ruminants caused by small ruminant morbillivirus (SRMV). In the present study, the full-length V gene of SRMV was constructed through site-directed mutagenesis from the P gene transcripts of the vaccine virus (Sungri/96 India) and expressed in a prokaryotic expression system. In animals, the seroconversion against this protein occurs from 14-days and is getting produced from 48 h in cell culture. An indirect ELISA developed using this protein has a relative sensitivity and relative specificity of 77.73% and 73.775%, respectively as compared to c-ELISA. In this ELISA, it was observed that most of the convalescent animals elicited higher level of antibodies than vaccinated animals.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest.
(© Indian Virological Society 2019.)
Databáze: MEDLINE
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