Land Management and Microbial Seed Load Effect on Rhizosphere and Endosphere Bacterial Community Assembly in Wheat.

Autor:	Kavamura VN; Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, United Kingdom., Robinson RJ; Plant Pathology Laboratory, RHS Garden Wisley, Woking, United Kingdom., Hayat R; Department of Soil Science and Soil and Water Conservation, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan., Clark IM; Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, United Kingdom., Hughes D; Computational and Analytical Sciences, Rothamsted Research, Harpenden, United Kingdom., Rossmann M; Laboratory of Environmental Microbiology, Embrapa Meio Ambiente, Jaguariúna, Brazil., Hirsch PR; Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, United Kingdom., Mendes R; Laboratory of Environmental Microbiology, Embrapa Meio Ambiente, Jaguariúna, Brazil., Mauchline TH; Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2019 Nov 15; Vol. 10, pp. 2625. Date of Electronic Publication: 2019 Nov 15 (Print Publication: 2019).
DOI:	10.3389/fmicb.2019.02625
Abstrakt:	Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant-microbe interactions for the development of novel microbial inoculants. (Copyright © 2019 Kavamura, Robinson, Hayat, Clark, Hughes, Rossmann, Hirsch, Mendes and Mauchline.)
Databáze:	MEDLINE
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