Correlations of the severity of diabetic retinopathy with EPO, Caspase-3 expression and oxidative stress.

Autor:	Tian M; Department of Ophthalmology, Fuxing Hospital, Capital Medical University, Beijing, China. kaloekaloe@sina.com., Liu S, Liu L, Zhang EK, Wang HW, Deng Y, Yue YK
Jazyk:	angličtina
Zdroj:	European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2019 Nov; Vol. 23 (22), pp. 9707-9713.
DOI:	10.26355/eurrev_201911_19532
Abstrakt:	Objective: To investigate the relationships of the severity of diabetic retinopathy with erythropoietin (EPO), Caspase-3 expression, and oxidative stress. Patients and Methods: A total of 20 patients with non-proliferative diabetic retinopathy hospitalized from January 2017 to January 2018 were enrolled as observation group 1, 20 patients with proliferative diabetic retinopathy were chosen as observation group 2, and 20 patients with idiopathic macular hole were selected as control group. After admission, patients received all necessary examinations and underwent vitrectomy during which vitreous and retinal tissues were taken, and venous blood was collected. Then, the content of EPO, Caspase-3, nitric oxide (NO), and malondialdehyde (MDA) was detected through enzyme-linked immunosorbent assay (ELISA), the messenger ribonucleic acid (mRNA) levels of EPO, Caspase-3, NO, and MDA were measured via quantitative Polymerase Chain Reaction (qPCR), and the severity of diabetic retinopathy was evaluated by diabetic retinopathy grading score. Results: Observation group 1 and 2 had significantly decreased the content of EPO (p<0.05) and overtly increased Caspase-3, NO, and MDA content (p<0.05) in comparison with control group. Compared with those in observation group 1, the EPO content was clearly lowered in observation group 2 (p<0.05), and the content of Caspase-3, NO, and MDA was evidently elevated (p<0.05). The diabetic retinopathy grading score was remarkably lower in control group than that in both observation group 1 and observation group 2 (p p<0.05), and it was significantly enhanced in observation group 2 compared with that in observation group 1 (p<0.05). Correlation analysis showed that the EPO content was negatively correlated with the severity of diabetic retinopathy, while the content of Caspase-3, NO, and MDA was positively related to the severity of diabetic retinopathy. Conclusions: The severity of diabetic retinopathy has a negative association with EPO and positive correlations with Caspase-3, NO, and MDA content.
Databáze:	MEDLINE
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