First report of malaria parasites in water buffalo in Nepal.

Autor: Kandel RC; Department of National Parks and Wildlife Conservation, Ministry of Forest and Environment, Government of Nepal, Kathmandu, Nepal., Shrestha M; Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal., Sadaula A; Biodiversity Conservation Center, National Trust for Nature Conservation- Chitwan, Nepal., Kc M; Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal., Maharjan J; Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal., Solanki GS; Department of Zoology, Mizoram University, India., Chalise MK; Department of Zoology, Tribhuvan University, Kirtipur, Nepal., Asada M; Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan., Kaneko O; Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan., Poudel RC; Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal. Electronic address: ramc_poudel@yahoo.com., Pandey K; Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Lalitpur, Nepal. Electronic address: pandey_kishor@hotmail.com.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2019 Dec; Vol. 18, pp. 100348. Date of Electronic Publication: 2019 Nov 06.
DOI: 10.1016/j.vprsr.2019.100348
Abstrakt: We present the first molecular-based report on ungulate malaria parasites from water buffalo in Nepal. Fifty-six blood samples were collected from different groups of water buffalo (wild, feral, and domestic) and PCR assays were conducted using Plasmodium spp. cytb specific primers. Two positive cases were detected, one each from feral and domestic individuals. Complete mitochondrial genome sequence (5987 bp) was obtained and examined for nucleotide variations. Sequence analysis revealed identity with type II water buffalo malaria parasites, reported previously, with one A to T nucleotide difference at position 5344. Prevalence, as well as possible economic impacts of water buffalo malaria, should be determined on a wider set of samples from buffalo across Nepal.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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