UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of the Spondias tuberosa Arruda Leaf and Root Hydroalcoholic Extracts.

Autor:	Thassya Lucas Dos Santos A; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Pereira Carneiro JN; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Pereira da Cruz R; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Lima Sales D; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Cosmo Andrade J; Brejo Santo Campus, Federal University of Cariri-UFCA, R. Olegario Emidio de Araujo, s/n, 63260-000 Centro, Brejo Santo-CE, Brazil., de Oliveira Almeida W; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Martins da Costa JG; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Riceli Vasconcelos Ribeiro P; Embrapa Tropical Agroindustry, R. Pernambuco, 2270, 60511-110 Pici, Fortaleza-CE, Brazil., Sousa de Brito E; Embrapa Tropical Agroindustry, R. Pernambuco, 2270, 60511-110 Pici, Fortaleza-CE, Brazil., Alves Batista FL; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Alves Magalhães FE; State University of Ceara-Tauá Campus, BR-020-Bezerra de Sousa, 63660-000 Tauá-CE, Brazil., Iriti M; Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, 20133 Milan, Italy., Morais-Braga MFB; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil., Coutinho HDM; Department of Biological Chemistry, Regional University of Cariri-URCA, Col Antonio Luis, 1161, 63105-000 Pimenta, Crato-CE, Brazil.
Jazyk:	angličtina
Zdroj:	Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2019 Nov 28; Vol. 8 (4). Date of Electronic Publication: 2019 Nov 28.
DOI:	10.3390/antibiotics8040240
Abstrakt:	The aim of this study was to identify and evaluate the chemical compositions and effects of the S. tuberosa leaf and root hydroalcoholic extracts (HELST and HERST) against different strains of Candida . Chemical analysis was performed by Ultra-Performance Liquid Chromatography Coupled to Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF). The Inhibitory Concentration of 50% of the growth (IC 50) as well as the intrinsic and combined action of the extracts with the antifungal fluconazole (FCZ) were determined by the microdilution method while the minimum fungicidal concentrations (MFCs) and the effect on fungal morphological transitions were analyzed by subculture and in humid chambers, respectively. From the preliminary phytochemical analysis, the phenols and flavonoids were the most abundant. The intrinsic IC 50 values for HELST ranged from 5716.3 to 7805.8 µg/mL and from 6175.4 to 51070.9 µg/mL for the HERST, whereas the combination of the extracts with fluconazole presented IC 50 values from 2.65 to 278.41 µg/mL. The MFC of the extracts, individually, for all the tested strains was ≥16384 µg/mL. When fluconazole was combined with each extract, the MFC against CA URM 5974 was reduced (HELST: 2048 and HERST: 4096 µg/mL). Synergism was observed against standard C. albicans (CA) and C. tropicalis (CT) strains and with the root extract against the CT isolate. The leaf extract inhibited the morphological transition of all strains while the root extract inhibited only CT strains.
Databáze:	MEDLINE
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