Junction Mapper is a novel computer vision tool to decipher cell-cell contact phenotypes.

Autor:	Brezovjakova H; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom., Tomlinson C; Bioinformatics Data Science Group, Faculty of Medicine, Imperial College London, London, United Kingdom., Mohd Naim N; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom., Swiatlowska P; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom., Erasmus JC; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom., Huveneers S; Department Medical Biochemistry, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Gorelik J; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom., Bruche S; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom., Braga VM; National Heart and Lung Institute, National Institutes of Health, London, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2019 Dec 03; Vol. 8. Date of Electronic Publication: 2019 Dec 03.
DOI:	10.7554/eLife.45413
Abstrakt:	Stable cell-cell contacts underpin tissue architecture and organization. Quantification of junctions of mammalian epithelia requires laborious manual measurements that are a major roadblock for mechanistic studies. We designed Junction Mapper as an open access, semi-automated software that defines the status of adhesiveness via the simultaneous measurement of pre-defined parameters at cell-cell contacts. It identifies contacting interfaces and corners with minimal user input and quantifies length, area and intensity of junction markers. Its ability to measure fragmented junctions is unique. Importantly, junctions that considerably deviate from the contiguous staining and straight contact phenotype seen in epithelia are also successfully quantified (i.e. cardiomyocytes or endothelia). Distinct phenotypes of junction disruption can be clearly differentiated among various oncogenes, depletion of actin regulators or stimulation with other agents. Junction Mapper is thus a powerful, unbiased and highly applicable software for profiling cell-cell adhesion phenotypes and facilitate studies on junction dynamics in health and disease. Competing Interests: HB, CT, NM, PS, JE, SH, JG, SB, VB No competing interests declared (© 2019, Brezovjakova et al.)
Databáze:	MEDLINE
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