Biological synthesis of silver nanoparticles using Rheum ribes and evaluation of their anticarcinogenic and antimicrobial potential: A novel approach in phytonanotechnology.

Autor: Aygün A; Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000 Kütahya, Turkey., Gülbağça F; Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000 Kütahya, Turkey., Nas MS; Department of Environmental, Faculty of Engineering, University of Igdir, 76000 Igdir, Turkey. Electronic address: mehmet.salih.nas@igdir.edu.tr., Alma MH; Department of Environmental, Faculty of Engineering, University of Igdir, 76000 Igdir, Turkey., Çalımlı MH; Medical Services and Technical Department of Tuzluca Vocational School, Igdir University, Igdir, Turkey. Electronic address: mharbi.calimli@igdir.edu.tr., Ustaoglu B; Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, University of Kastamonu, Kastamonu, Turkey., Altunoglu YC; Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, University of Kastamonu, Kastamonu, Turkey., Baloğlu MC; Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, University of Kastamonu, Kastamonu, Turkey., Cellat K; Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000 Kütahya, Turkey., Şen F; Sen Research Group, Department of Biochemistry, University of Dumlupınar, 43000 Kütahya, Turkey. Electronic address: fatih.sen@dpu.edu.tr.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2020 Feb 05; Vol. 179, pp. 113012. Date of Electronic Publication: 2019 Nov 26.
DOI: 10.1016/j.jpba.2019.113012
Abstrakt: This paper reports the anticarcinogenic and antimicrobial properties of silver nanoparticles (Ag NPs) obtained by green synthesis using the extract of Rheum ribes (R. ribes), a medicinal plant. For the synthesis of Ag NPs, the ethanolic extracts of R. ribes were used as a reducing as well as the stabilizing agent. For the characterization of Ag NPs, advanced analytical methods such as transmission electron microscopy (TEM), X-Ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and UV-vis spectrophotometry were performed. The synthesized Ag NPs obtained from R. ribes were evaluated as a cytotoxic agent against MDA-MB-231 breast carcinoma cell line. The IC 50 values of the nanoparticles were ranged from 165 to 99 μg/mL against MDA-MB 231 cell line for 24 h and 48 h, respectively. The results show that the use of Ag NPs at low concentrations show the toxic effect in the cancer cells. In addition, the results of experiments on gram-positive (Staphylococcus aureus (S. aureus), Methicillin-resistant Staphylococcus aureus (MRSA) and Bacillus subtilis (B. subtilis)) and gram-negative (Escherichia coli (E. coli)) bacteria showed that the Ag NPs had high antimicrobial activity. The results suggest that Ag NPs can be developed as potential anticancer and antibacterial agents.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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