Evaluation of the Unyvero i60 ITI® multiplex PCR for infected chronic leg ulcers diagnosis.

Autor: Reissier S; Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France. Electronic address: sophie.reissier@gmail.com., Lazareth I; Vascular Medicine and Phlebology Service, Saint-Joseph Hospital, Paris, France., Adjiman L; Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France., Couzigou C; Antimicrobial Stewardship Team, Saint-Joseph Hospital, Paris, France., Vidal B; Antimicrobial Stewardship Team, Saint-Joseph Hospital, Paris, France., Mizrahi A; Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France., Nguyen Van JC; Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France., Pilmis B; Antimicrobial Stewardship Team, Saint-Joseph Hospital, Paris, France., Le Monnier A; Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France., Lourtet-Hascoët J; Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2020 Jan; Vol. 168, pp. 105796. Date of Electronic Publication: 2019 Nov 29.
DOI: 10.1016/j.mimet.2019.105796
Abstrakt: Objectives: Unyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in chronic leg ulcer (CLU) infections.
Methods: A prospective study, part of the interventional-blinded randomized study "ulcerinfecte" (NCT02889926), was conducted at Saint Joseph Hospital in Paris. Fifty patients with a suspicion of infected CLU were included between February 2017 and September 2018. Conventional bacteriology and mPCR were performed simultaneously on deep skin biopsies.
Results: Staphylococcus aureus and Pseudomonas aeruginosa were the most detected pathogens. Regarding the global sensitivity, mPCR is not overcome to the standard culture. Anaerobes and slow growing bacteria were detected with a higher sensitivity rate by mPCR than standard culture.
Conclusion: Unyvero i60 ITI multiplex PCR detected rapidly pathogenic bacteria in infected CLU especially anaerobes and slow growing bacteria and was particularly effective for patients previously treated with antibiotics.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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