Microenvironment-Responsive Small-Molecule Probe for Pulmonary Fibrosis Detection.

Autor: Dong Y; State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203 , China.; University of Chinese Academy of Sciences , Beijing 100049 , China., Li XR; College of Pharmaceutical Sciences , Zhejiang University , Hangzhou 310058 , China., Chen Q; Department of Pulmonology , Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine , Shanghai , 201203 , China., Guo RY; College of Pharmaceutical Sciences , Zhejiang University , Hangzhou 310058 , China., Tang BX; State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203 , China.; University of Chinese Academy of Sciences , Beijing 100049 , China., Kan WJ; State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203 , China., Zhang W; Department of Pulmonology , Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine , Shanghai , 201203 , China., Hu Y; College of Pharmaceutical Sciences , Zhejiang University , Hangzhou 310058 , China., Li J; State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203 , China., Zang Y; State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203 , China., Li X; College of Pharmaceutical Sciences , Zhejiang University , Hangzhou 310058 , China.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2020 Jan 07; Vol. 92 (1), pp. 699-706. Date of Electronic Publication: 2019 Dec 13.
DOI: 10.1021/acs.analchem.9b02264
Abstrakt: Pulmonary fibrosis (PF) is a fatal disease with increasing prevalence. Nonradioactive and noninvasive diagnosis of PF at an early stage can improve the prognosis but represents a daunting challenge. Up-regulation of nitric oxide (NO) is a typical microenvironmental feature of PF. Here, we report a small-molecule probe, PNO1 , that can fluorogenically sense this microenvironmental feature for PF diagnosis. We demonstrate that PNO1 fluorescence is 6-fold higher in PF-diseased mice lungs than in normal-control groups. In addition to this in vivo result, PNO1 can also be applied in vitro to detect PF-diseased cells and ex vivo to detect PF-diseased tissues from clinical patients. These results highlight PNO1 as a complement to the traditional immunostaining-based methods for PF detection to facilitate quick screening for anti-PF drug candidates.
Databáze: MEDLINE
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