Detection of Small Subsets of CD4 + Lymphocytes with SmartFlare Nanoprobes.

Autor: Toptygina AP; G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being, Moscow, Russia. toptyginaanna@rambler.ru., Zakirov RS; National Medical Research Center for Children's Health, Ministry of Health of the Russian Federation, Moscow, Russia., Kapitanova KS; G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being, Moscow, Russia., Semikina EL; National Medical Research Center for Children's Health, Ministry of Health of the Russian Federation, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2019 Dec; Vol. 168 (2), pp. 270-274. Date of Electronic Publication: 2019 Nov 29.
DOI: 10.1007/s10517-019-04689-5
Abstrakt: SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4 + lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4 + T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.
Databáze: MEDLINE
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