Nanoscale View of Amyloid Photodynamic Damage.

Autor:	Bondia P; Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia) , C/Faraday 9 , Madrid 28049 , Spain., Torra J; Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia) , C/Faraday 9 , Madrid 28049 , Spain., Tone CM; Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia) , C/Faraday 9 , Madrid 28049 , Spain., Sawazaki T; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku , Tokyo 113-0033 , Japan., Del Valle A; Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia) , C/Faraday 9 , Madrid 28049 , Spain., Sot B; Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia) , C/Faraday 9 , Madrid 28049 , Spain., Nonell S; Institut Químic de Sarrià , Universitat Ramon Llull , Via Augusta 390 , Barcelona 08017 , Spain., Kanai M; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku , Tokyo 113-0033 , Japan., Sohma Y; Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku , Tokyo 113-0033 , Japan., Flors C; Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia) , C/Faraday 9 , Madrid 28049 , Spain.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2020 Jan 15; Vol. 142 (2), pp. 922-930. Date of Electronic Publication: 2019 Dec 12.
DOI:	10.1021/jacs.9b10632
Abstrakt:	A combination of time-resolved optical spectroscopy and nanoscale imaging has been used to study the complex binding to amyloids of a photocatalyst that selectively photo-oxygenates pathogenic aggregates, as well as the consequences of its irradiation. Correlative atomic force microscopy (AFM) and fluorescence microscopy reveals topography-dependent binding of the dye to model β-lactoglobulin fibers, which may also explain the observed difference in their response to photodegradation. We provide direct evidence of the photosensitization of singlet oxygen by the photocatalyst bound to amyloid fibers by direct detection of its NIR phosphorescence. The effect of singlet oxygen at the molecular level brings about nanoscale morphological changes that can be observed with AFM at the single-fiber level. We also find differential response of two α-synuclein mutants to photodamage, which can be rationalized by the presence of amino acids susceptible to photo-oxygenation. Overall, our results help to unravel some of the complexity associated with highly heterogeneous amyloid populations and contribute to the development of improved phototherapeutic strategies for amyloid-related disorders.
Databáze:	MEDLINE
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