Implementation of research on potential drug target cloning and characterization in a biochemistry laboratory.

Autor: Wu C; Division of Natural Sciences, Mount Marty College, Yankton, South Dakota., McCune TS; Division of Natural Sciences, Mount Marty College, Yankton, South Dakota.
Jazyk: angličtina
Zdroj: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology [Biochem Mol Biol Educ] 2020 Mar; Vol. 48 (2), pp. 108-117. Date of Electronic Publication: 2019 Nov 29.
DOI: 10.1002/bmb.21319
Abstrakt: An approach for incorporating preliminary drug discovery research into a biochemistry laboratory is described. During a total of 42 hr (one 3-hr laboratory section a week for 14 weeks), students were exposed to bioinformatics; molecular cloning; protein expression, purification, and characterization; and enzymatic kinetic assays. This research-oriented laboratory not only includes the standard elements of common undergraduate biochemistry laboratory manuals but also emphasizes the logical connections among biochemistry laboratory techniques. Moreover, this approach exposed students to laboratory research and the concept of drug discovery.
(© 2019 International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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