| Autor: |
Silverman AD; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Karim AS; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.; Simpson Querrey Institute, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu. |
| Abstrakt: |
Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits. |
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