Determination of antimicrobial susceptibility and biofilm production in Staphylococcus aureus isolated from white coats of health university students.

Autor: Batista IR; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Prates ACL; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Santos BS; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Araújo JCC; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Bonfim YCO; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Pimenta Rodrigues MV; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Morceli G; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Polettini J; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Cavalleri AC; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Winkelstroter LK; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil., Pereira VC; Universidade do Oeste Paulista - UNOESTE, Rua José Bongiovani, 700 - Cidade Universitária, Presidente Prudente, SP, CEP 19050-920, Brazil. valeriacataneli@gmail.com.
Jazyk: angličtina
Zdroj: Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2019 Nov 28; Vol. 18 (1), pp. 37. Date of Electronic Publication: 2019 Nov 28.
DOI: 10.1186/s12941-019-0337-6
Abstrakt: This study aimed at detecting Staphylococcus aureus from white coats of college students and characterizing antimicrobial susceptibility and biofilm production. Bacterial samples (n = 300) were obtained from white coats of 100 college students from August 2015 to March 2017 S. aureus was isolated and it´s resistance profile was assessed by antimicrobial disk-diffusion technique, screening for methicillin-resistant Staphylococcus aureus (MRSA), detection of mecA gene by PCR, and determination of staphylococcal cassette chromosome mec (SCCmec) by multiplex PCR. Congo red agar (CRA) and icaA and icaD genes by PCR were used for biofilm characterization. S. aureus was identified in 45.0% of samples. Resistance of S. aureus sample to antimicrobial was seen for penicillin (72.59%), erythromycin (51.85%), cefoxitin (20.74%), oxacillin (17.04%), clindamycin (14.81%) and levofloxacin (5.18%). MRSA was detected in 53.3% of the samples with SCCmec I (52.8%), SCCmec III (25%) and SCCmec IV (11.1%). Biofilm production was observed in 94.0% S. aureus samples. These data show that biosafety measures need to be enhanced in order to prevent dissemination of multiresistant and highly adhesive bacteria across other university settings, relatives, and close persons.
Databáze: MEDLINE
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