| Autor: |
Suriani Ribeiro M; Department of Biochemistry and Cellular Biology, Biological Sciences Institute, Campus Samambaia, Federal University of Goiás (UFG), Goiânia, Goiás 74090-900, Brazil., Graciano de Paula R; Department of Physiological Sciences, Health Sciences Centre, Federal University of Espirito Santo, Vitoria ES 29047-105, Brazil., Raquel Voltan A; Department of Biochemistry and Cellular Biology, Biological Sciences Institute, Campus Samambaia, Federal University of Goiás (UFG), Goiânia, Goiás 74090-900, Brazil., de Castro RG; Department of Biochemistry and Cellular Biology, Biological Sciences Institute, Campus Samambaia, Federal University of Goiás (UFG), Goiânia, Goiás 74090-900, Brazil., Carraro CB; Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto 14049-900, Brazil., José de Assis L; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, Ribeirão Preto CEP 14040-903, Brazil., Stecca Steindorff A; Department of Cell Biology, University of Brasília, Campus Darcy Ribeiro, Biological Sciences Institute, Brasília CEP 70.910-900, Brazil., Goldman GH; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, Ribeirão Preto CEP 14040-903, Brazil., Silva RN; Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto 14049-900, Brazil., Ulhoa CJ; Department of Biochemistry and Cellular Biology, Biological Sciences Institute, Campus Samambaia, Federal University of Goiás (UFG), Goiânia, Goiás 74090-900, Brazil., Neves Monteiro V; Campus of Exact Sciences and Technologies, Campus Henrique Santillo, State University of Goiás (UEG), BR 153, Km 98, Anápolis 75000-000, Brazil. |
| Abstrakt: |
Trichoderma species are known for their ability to produce lytic enzymes, such as exoglucanases, endoglucanases, chitinases, and proteases, which play important roles in cell wall degradation of phytopathogens. β-glucanases play crucial roles in the morphogenetic-morphological process during the development and differentiation processes in Trichoderma species, which have β-glucans as the primary components of their cell walls. Despite the importance of glucanases in the mycoparasitism of Trichoderma spp., only a few functional analysis studies have been conducted on glucanases. In the present study, we used a functional genomics approach to investigate the functional role of the gluc31 gene, which encodes an endo-β-1,3-glucanase belonging to the GH16 family in Trichoderma harzianum ALL42. We demonstrated that the absence of the gluc31 gene did not affect the in vivo mycoparasitism ability of mutant T. harzianum ALL42; however, gluc31 evidently influenced cell wall organization. Polymer measurements and fluorescence microscopy analyses indicated that the lack of the gluc31 gene induced a compensatory response by increasing the production of chitin and glucan polymers on the cell walls of the mutant hyphae. The mutant strain became more resistant to the fungicide benomyl compared to the parental strain. Furthermore, qRT-PCR analysis showed that the absence of gluc31 in T. harzianum resulted in the differential expression of other glycosyl hydrolases belonging to the GH16 family, because of functional redundancy among the glucanases. |
