Determination of S-adenosylmethionine, S-adenosylhomocysteine, and methylthioadenosine in urine using solvent-modified micellar electrokinetic chromatography.

Autor: Ivanov AV; Department of Molecular and Cell Pathophysiology, Federal State Budgetary Scientific Institution 'Institute of General Pathology and Pathophysiology', Moscow, Russia., Kruglova MP; I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Virus ED; Department of Molecular and Cell Pathophysiology, Federal State Budgetary Scientific Institution 'Institute of General Pathology and Pathophysiology', Moscow, Russia., Bulgakova PO; Department of Molecular and Cell Pathophysiology, Federal State Budgetary Scientific Institution 'Institute of General Pathology and Pathophysiology', Moscow, Russia., Vital'evich Grachev S; I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Kubatiev AA; Department of Molecular and Cell Pathophysiology, Federal State Budgetary Scientific Institution 'Institute of General Pathology and Pathophysiology', Moscow, Russia.; Russian Medical Academy of Continuous Professional Education, Moscow, Russia.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2020 Feb; Vol. 41 (3-4), pp. 209-214. Date of Electronic Publication: 2019 Dec 06.
DOI: 10.1002/elps.201900364
Abstrakt: A new approach for direct determination of S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and methylthioadenosine (MTA) in urine was developed based on MEKC by using SDS modified with isobutanol in the presence of PEG-300. Analytes were first extracted with grafted phenylborononic acid. Using a 50 µm internal diameter silica capillary of 32 cm total length filled with 0.05 M SDS, 0.05 M H 3 PO 4 , 5% (v/v) isobutanol, and 10% (v/v) PEG-300, LOQ of 0.15 µM for SAM and SAH, and 0.2 µM for MTA was reached. Accuracy was 92% for MTA, 109% for SAH, and 105% for SAM, intra- and interday imprecision were <2.5 and ≤3%, respectively. The total time of analysis for one sample was 10 min. Analysis of 30 urine samples from healthy volunteers showed that the median SAM and SAH levels were 12.1 and 0.73 µM, respectively. MTA levels, which were determined in urine for the first time (according to our data), were 0.43 µM, and these values correlated well with the SAM level (r = 0.748, p < 0.01).
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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