| Autor: |
Galo JM; Instituto Federal de Educação, Ciência e Tecnologia de Rondônia - IFRO, Campus Ariquemes, Rodovia RO-257, Km 13, CEP 76878-899, Ariquemes, RO, Brasil., Streit-Jr DP; Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul - UFRGS, Av. Bento Gonçalves, 7712, CEP 91540-000, Porto Alegre, RS, Brasil., Corcini CD; Faculdade de Veterinária, Universidade Federal de Pelotas - UFPEL, Rua Gomes Carneiro, 01, Centro, CEP 96010-900, Pelotas, RS, Brasil., Varela-Jr AS; Setor de Morfologia-Histologia, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália, s/n, Campus Carreros, Prédio 06, CEP 96203-000, Rio Grande, RS, Brasil., Jardim RD; Setor de Morfologia-Histologia, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Av. Itália, s/n, Campus Carreros, Prédio 06, CEP 96203-000, Rio Grande, RS, Brasil., Ribeiro RP; Departamento de Zootecnia, Universidade Estadual de Maringá - UEM, Av. Colombo, 5790, CEP 87030-121, Bairro Zona 07, Maringá, PR, Brasil. |
| Abstrakt: |
The aim of this study was to evaluate the association between proteins in the seminal plasma of tambaqui Colossoma macropomum (Cuvier, 1818) with seminal quality indicators after thawing. The semen was cryopreserved with a dilution based on BTS with 8% DMSO. A 200 µL sample of semen from each animal was diluted in 800 µL BTS, centrifuged at 800 rpm, and the supernatant was cryopreserved to further analyze of the protein profile of seminal plasma through one-dimensional electrophoresis (SDS-PAGE). After 15 days of cryopreservation, a cryopreserved semen straw was thawed to analyze both qualitative and quantitative parameters. When considering all collections, the SDS-PAGE identified 15 protein bands in the seminal plasma of tambaqui. When the interaction (presence or absence) between proteins observed in the seminal plasma and the post thawed spermatic parameters was evaluated, we observed a great influence of the presence of proteins on spermatic quality. A greater (P<0.05) fertilization rate was observed with the presence of proteins 12, 34, 44, 85, and 90 kDa. Proteins in seminal plasma of tambaqui influenced the spermatic quality after thawing, and thus, they can be utilized as an indicator of sperm quality, especially the proteins with a molecular weight ≤ 50 kDa. |
