| Autor: |
Nascimento ACDS; Universidade Federal do Mato Grosso do Sul - UFMS, School of Medicine , Post-graduate Program of Health and Development of the Center Western Region , Campo Grande , MS , Brazil ., Nocetti MC; Universidade Federal do Mato Grosso do Sul - UFMS, Institute of Biosciences , Campo Grande , MS , Brazil ., Lugo LZA; Universidade Federal do Mato Grosso do Sul - UFMS, School of Medicine , Post-graduate Program of Infectious and Parasitic Diseases , Campo Grande , MS , Brazil ., Jacob CMB; Universidade Federal do Mato Grosso do Sul - UFMS, School of Medicine , Post-graduate Program of Infectious and Parasitic Diseases , Campo Grande , MS , Brazil ., Machado AP; Universidade Federal do Mato Grosso do Sul - UFMS, Institute of Biosciences, Multicentric Postgraduate Program in Biochemistry and Molecular Biology, Campo Grande, MS, Brazil., Padovani CTJ; Universidade Federal do Mato Grosso do Sul - UFMS, Institute of Biosciences , Campo Grande , MS , Brazil ., Ferreira AMT; Universidade Federal do Mato Grosso do Sul - UFMS, Institute of Biosciences , Campo Grande , MS , Brazil ., Fernandes CEDS; Universidade Federal do Mato Grosso do Sul - UFMS, Institute of Biosciences , Campo Grande , MS , Brazil ., Tozetti IA; Universidade Federal do Mato Grosso do Sul - UFMS, Institute of Biosciences, Multicentric Postgraduate Program in Biochemistry and Molecular Biology, Campo Grande, MS, Brazil. |
| Abstrakt: |
Human Papillomavirus (HPV) has considerable tropism for epithelial and mucosal tissues and can therefore be found in several anatomical sites, including the oral cavity. This study aimed to investigate the presence of HPV-DNA and the most frequent viral types in patients using full dentures, compare to patients not using full dentures and to associate its presence with socio-epidemiological and behavioral factors. The study consisted of 90 patients with or without full dentures at the time of collection, treated at a public dental clinic. The samples were obtained by exfoliating the oral cavity, and analyzed for HPV-DNA using the nested PCR with PGMY09/11 (450-bp), and general primers GP5+/GP6+ (150-bp). Genotyping was performed by specific-type PCR to HPV 6, 11, 16, 18, 31, 33, and 45; and Restriction Fragment Length Polymorphism (RFLP). Pearson's Chi-square test (x 2 ) or Fisher's exact test were applied and significant variables in these tests were analyzed by multinomial logistic regression analysis to estimate odds ratio (OR). HPV-DNA was detected in 27.7% of samples and, among those obtained from patients using full dentures, positivity for HPV-DNA was 41.9% (p = 0.025). The most frequent viral types were low-risk HPV 6 and 11, and high-risk HPV 31 and 45. Patients who used full dentures had an odds ratio of 2.1 to be positive for HPV DNA. Our results indicate the need for periodic dental follow-up of patients with full dentures in order to preserve the basic conditions of oral health, and also to monitor the appearance of lesions with malignant potential. |
