| Autor: |
Shim AL; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia., Kamkin AG; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia. Andre.Gleb.Kamkin@gmail.com., Kamkina OV; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia., Kazanskii VE; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia., Mitrokhin VM; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia., Bilichenko AS; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia., Filatova TS; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.; Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia., Abramochkin DV; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.; Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia. |
| Abstrakt: |
The whole-cell patch-clamp technique was used to examine the effect of gadolinium Gd 3+ (a non-specific blocker of mechanically gated current I MGCh , a component of late current I L ) on ionic currents in insolated rat ventricular cardiomyocytes alone and in combination with the blockers of L-type calcium currents (I CaL ) nifedipine (10 μM) or verapamil (1 μM). In K + in /K + out or Cs + in /Cs + out media, blockade of I CaL produced no effect on I L at negative potentials, but inhibited I L at positive ones. In K + in /K + out medium, Gd 3+ (5 μM) decreased the net persistent current (I np ) at -45 mV from 198.6±6.4 to 96.7±9.5 pA over 15 min. Gd 3+ alone or in combination with I CaL blockers shifted the reversal potential of I L to more negative values. At negative potentials, Gd 3+ decreased I K1 and inward current including I MGCh . At positive potentials, Gd 3+ alone or in combination with I CaL blockers decreased I L . When applied for 15 min in Cs + in /Cs + out medium at -45 mV, Gd 3+ produced no effect on net current and inward and outward components of I L . Thus, Gd 3+ can be viewed as a specific blocker of I MGCh only in Cs + medium. |
Full text from SpringerLink