Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT.

Autor:	Bornes L; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands., van Scheppingen RH; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands., Beerling E; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands., Schelfhorst T; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands., Ellenbroek SIJ; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands., Seinstra D; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands. Electronic address: d.seinstra@amsterdamumc.nl., van Rheenen J; Division of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, the Netherlands. Electronic address: j.v.rheenen@nki.nl.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2019 Nov 26; Vol. 29 (9), pp. 2565-2569.e3.
DOI:	10.1016/j.celrep.2019.10.107
Abstrakt:	Epithelial-to-mesenchymal transition (EMT) has long been thought to be crucial for metastasis. Recently a study challenged this idea by demonstrating that metastases were seeded by tumor cells that were not marked by an EMT lineage-tracing reporter on the basis of the expression of the mesenchymal marker fsp1. However, the results of this study and their interpretation are under debate. Here, we combine the lineage-tracing reporter with our real-time EMT-state reporter and show that the fsp1-based EMT lineage-tracing reporter does not mark all disseminating mesenchymal cells with metastatic potential. Our findings demonstrate that fsp1-mediated lineage tracing does not allow any conclusions about the requirement of EMT for metastasis. Instead our data are fully consistent with previous reports that EMT is not a binary phenomenon but rather a spectrum of cellular states. (Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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