| Autor: |
Song C; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science; Biomaterials Research Center, School of Biomedical Engineering , Southern Medical University , Guangzhou , Guangdong 510515 , China.; Department of Mechanical Engineering, Faculty of Engineering, Department of Biochemistry and Biomedical Genetics, Faculty of Medicine , University of Manitoba , Winnipeg , Manitoba R3T 2N2 , Canada., Zhang X; Department of Mechanical Engineering, Faculty of Engineering, Department of Biochemistry and Biomedical Genetics, Faculty of Medicine , University of Manitoba , Winnipeg , Manitoba R3T 2N2 , Canada., Wang L; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science; Biomaterials Research Center, School of Biomedical Engineering , Southern Medical University , Guangzhou , Guangdong 510515 , China., Wen F; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science; Biomaterials Research Center, School of Biomedical Engineering , Southern Medical University , Guangzhou , Guangdong 510515 , China., Xu K; Department of Mechanical Engineering, Faculty of Engineering, Department of Biochemistry and Biomedical Genetics, Faculty of Medicine , University of Manitoba , Winnipeg , Manitoba R3T 2N2 , Canada., Xiong W; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science; Biomaterials Research Center, School of Biomedical Engineering , Southern Medical University , Guangzhou , Guangdong 510515 , China., Li C; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science; Biomaterials Research Center, School of Biomedical Engineering , Southern Medical University , Guangzhou , Guangdong 510515 , China., Li B; Department of Orthopedics, School of Medicine , West Virginia University , Morgantown , West Virginia 26506 , United States., Wang Q; Department of Civil and Environmental Engineering , Shantou University , Shantou , Guangdong 515063 , China., Xing MMQ; Department of Mechanical Engineering, Faculty of Engineering, Department of Biochemistry and Biomedical Genetics, Faculty of Medicine , University of Manitoba , Winnipeg , Manitoba R3T 2N2 , Canada., Qiu X; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science; Biomaterials Research Center, School of Biomedical Engineering , Southern Medical University , Guangzhou , Guangdong 510515 , China. |
| Abstrakt: |
Designing scaffolds with persistent elasticity and conductivity to mimic microenvironments becomes a feasible way to repair cardiac tissue. Injectable biomaterials for cardiac tissue engineering have demonstrated the ability to restore cardiac function by preventing ventricular dilation, enhancing angiogenesis, and improving conduction velocity. However, limitations are still among them, such as poor mechanical stability, low conductivity, and complicated procedure. Here, we developed thermal plastic poly(glycolic acid) surgical suture and mussel-inspired conductive particle's adhesion into a highly elastic, conductive spring-like coils. The polypyrrole (PPy)-coated biospring acted as an electrode and then was assembled into a solid-state supercapacitor. After being injected through a syringe needle (0.33 mm inner diameter), the tangled coils formed an elastically conductive three-dimensional (3-D) network to modulate cardiac function. We found that cardiomyocytes (CMs) grew along the spring coils' track with elongated morphologies and formed highly oriented sarcomeres. The biospring enhanced the CMs' maturation in synchronous contraction accompanied by high expressions of cardiac-specific proteins, α-actinin, and connexin 43 (cx43). After the elastic, conductive biosprings were injected into the myocardial infarction (MI) area, the left ventricular fractional shortening was improved by about 12.6% and the infarct size was decreased by about 34%. Interestingly, the spring can be utilized as a sensor to measure the CMs' contractile force, which was 1.57 × 10 -3 ± 0.26 × 10 -3 mN (∼4.1 × 10 6 cells). Accordingly, this study highlights an injectable biospring to form a tangled conductive 3-D network in vivo for MI repair. |
