Lubricant-Infused PET Grafts with Built-In Biofunctional Nanoprobes Attenuate Thrombin Generation and Promote Targeted Binding of Cells.

Autor: Badv M; School of Biomedical Engineering, McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada., Weitz JI; School of Biomedical Engineering, McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada.; Thrombosis & Atherosclerosis Research Institute, 237 Barton Street East, L8L 2X2, Hamilton, Ontario, Canada.; Department of Medicine, McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada., Didar TF; School of Biomedical Engineering, McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada.; Department of Mechanical Engineering, McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada.; Michael G. DeGroote Institute for Infectious Disease Research (IIDR), McMaster University, 1280 Main Street West, L8S 4L8, Hamilton, Ontario, Canada.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2019 Dec; Vol. 15 (51), pp. e1905562. Date of Electronic Publication: 2019 Nov 27.
DOI: 10.1002/smll.201905562
Abstrakt: New surface coatings that enhance hemocompatibility and biofunctionality of synthetic vascular grafts such as expanded poly(tetrafluoroethylene) (ePTFE) and poly(ethylene terephthalate) (PET) are urgently needed. Lubricant-infused surfaces prevent nontargeted adhesion and enhance the biocompatibility of blood-contacting surfaces. However, limited success has been made in incorporating biofunctionality onto these surfaces and generating biofunctional lubricant-infused coatings that both prevent nonspecific adhesion and enhance targeted binding of biomolecules remains a challenge. Here, a new generation of fluorosilanized lubricant-infused PET surfaces with built-in biofunctional nanoprobes is reported. These surfaces are synthesized by starting with a self-assembled monolayer of fluorosilane that is partially etched using plasma modification technique, thereby creating a hydroxyl-terminated fluorosilanized PET surface. Simultaneously, silanized nanoprobes are produced by amino-silanizing anti-CD34 antibody in solution and directly coupling the anti-CD34-aminosilane nanoprobes onto the hydroxyl terminated, fluorosilanized PET surface. The PET surfaces are then lubricated, creating fluorosilanized biofunctional lubricant-infused PET substrates. Compared with unmodified PET surfaces, the designed biofunctional lubricant-infused PET surfaces significantly attenuate thrombin generation and blood clot formation and promote targeted binding of endothelial cells from human whole blood.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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