Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice.

Autor: Panis C; Laboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, Francisco Beltrão, Paraná 85605-010, Brazil., Victorino VJ; Laboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, Francisco Beltrão, Paraná 85605-010, Brazil.; Laboratório de Imunopatologia Experimental, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, 86051-970 Paraná, Brazil., Tatakihara VLH; Laboratório de Imunopatologia Experimental, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, 86051-970 Paraná, Brazil., Cecchini R; Laboratório de Patofisiologia e Radicais Livres, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, Brazil., Rizzo LV; Hospital Israelita Albert Einstein, Avenida Albert Einstein 627-701, Subsolo Bloco A., 05651-901 São Paulo, São Paulo, Brazil., Yamauchi LM; Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, Brazil., Yamada-Ogatta SF; Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, Brazil., Martins-Pinge MC; Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, Brazil., Pinge-Filho P; Laboratório de Imunopatologia Experimental, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, 86051-970 Paraná, Brazil.
Jazyk: angličtina
Zdroj: Mediators of inflammation [Mediators Inflamm] 2019 Oct 24; Vol. 2019, pp. 5091630. Date of Electronic Publication: 2019 Oct 24 (Print Publication: 2019).
DOI: 10.1155/2019/5091630
Abstrakt: Infection with the protozoan Trypanosoma cruzi causes Chagas disease and consequently leads to severe inflammatory heart condition; however, the mechanisms driving this inflammatory response have not been completely elucidated. Nitric oxide (NO) is a key mediator of parasite killing in T. cruzi -infected mice, and previous studies have suggested that leukotrienes (LTs) essentially regulate the NO activity in the heart. We used infected 5-lipoxygenase-deficient mice (5-LO -/- ) to explore the participation of nitric oxide synthase isoforms, inducible (iNOS) and constitutive (cNOS), in heart injury, cytokine profile, and oxidative stress during the early stage of T. cruzi infection. Our evidence suggests that the cNOS of the host is involved in the resistance of 5-LO -/- mice during T. cruzi infection. iNOS inhibition generated a remarkable increase in T. cruzi infection in the blood and heart of mice, whereas cNOS inhibition reduced cardiac parasitism (amastigote nests). Furthermore, this inhibition associates with a higher IFN- γ production and lower lipid peroxidation status. These data provide a better understanding about the influence of NO-interfering therapies for the inflammatory response toward T. cruzi infection.
Competing Interests: The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.
(Copyright © 2019 Carolina Panis et al.)
Databáze: MEDLINE
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