Bacillus safensis JG-B5T affects the fate of selenium by extracellular production of colloidally less stable selenium nanoparticles.

Autor: Fischer S; Helmholtz-Zentrum Dresden - Rossendorf e. V., Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany., Krause T; Technische Universität Dresden, Institute of Microbiology, Chair of Molecular Biotechnology, 01062 Dresden, Germany., Lederer F; Helmholtz-Zentrum Dresden - Rossendorf e. V., Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, 01328 Dresden, Germany., Merroun ML; University of Granada, Department of Microbiology, Campus Fuentenueva, E-18071 Granada, Spain., Shevchenko A; Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany., Hübner R; Helmholtz-Zentrum Dresden - Rossendorf e. V., Institute of Ion Beam Physics and Materials Research, Bautzner Landstrasse 400, 01328 Dresden, Germany., Firkala T; Helmholtz-Zentrum Dresden - Rossendorf e. V., Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, 01328 Dresden, Germany., Stumpf T; Helmholtz-Zentrum Dresden - Rossendorf e. V., Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany., Jordan N; Helmholtz-Zentrum Dresden - Rossendorf e. V., Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany. Electronic address: n.jordan@hzdr.de., Jain R; Helmholtz-Zentrum Dresden - Rossendorf e. V., Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, 01328 Dresden, Germany. Electronic address: r.jain@hzdr.de.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2020 Feb 15; Vol. 384, pp. 121146. Date of Electronic Publication: 2019 Sep 03.
DOI: 10.1016/j.jhazmat.2019.121146
Abstrakt: Understanding the impact of microorganisms on the mobility of selenium (Se) is important for predicting the fate of toxic Se in the environment and improving wastewater treatment technologies. The bacteria strain Bacillus safensis JG-B5T, isolated from soil in a uranium mining waste pile, can influence the Se speciation in the environment and engineered systems. However, the mechanism and conditions of this process remain unknown. This study found that the B. safensis JG-B5T is an obligate aerobic microorganism with an ability to reduce 70% of 2.5 mM selenite to produce red spherical biogenic elemental selenium nanoparticles (BioSeNPs). Only extracellular production of BioSeNPs was observed using transmission electron microscopy. The two-chamber reactor experiments, genome analysis and corona proteins identified on BioSeNPs suggested that the selenite reduction process was primarily mediated through membrane-associated proteins, like succinate dehydrogenase. Extracellular presence and low colloidal stability of BioSeNPs as indicated by ζ-potential measurements, render B. safensis JG-B5T an attractive candidate in wastewater treatment as it provides easy way of recovering Se while maintaining low Se discharge. As this microorganism decreases Se mobility, it will affect Se bioavailability in the environment and decreases its toxicity.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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