| Autor: |
Halicki PCB; Núcleo de Pesquisa em Microbiologia Médica (NUPEMM), Faculdade de Medicina da Universidade Federal do Rio Grande, FURG, Rio Grande, Brazil., Radin V; Núcleo de Pesquisa em Microbiologia Médica (NUPEMM), Faculdade de Medicina da Universidade Federal do Rio Grande, FURG, Rio Grande, Brazil., von Groll A; Núcleo de Pesquisa em Microbiologia Médica (NUPEMM), Faculdade de Medicina da Universidade Federal do Rio Grande, FURG, Rio Grande, Brazil., Nora MV; Fundação Oswaldo Cruz, Instituto De Tecnologia Em Fármacos, Farmanguinhos, Rio De Janeiro, Brazil., Pinheiro AC; Fundação Oswaldo Cruz, Instituto De Tecnologia Em Fármacos, Farmanguinhos, Rio De Janeiro, Brazil., da Silva PEA; Núcleo de Pesquisa em Microbiologia Médica (NUPEMM), Faculdade de Medicina da Universidade Federal do Rio Grande, FURG, Rio Grande, Brazil., Ramos DF; Núcleo de Pesquisa em Microbiologia Médica (NUPEMM), Faculdade de Medicina da Universidade Federal do Rio Grande, FURG, Rio Grande, Brazil. |
| Abstrakt: |
In the last 15 years, Acinetobacter baumannii has received special attention, mainly due to several resistance mechanisms and high rates of morbimortality. The ability to form biofilms contributes to the persistence of this microorganism in the hospital environment and facilitates the occurrence of nosocomial infections. Several studies have highlighted the pharmacological relevance of pyridines in the treatment and control of infectious diseases and others have related the anti- A. baumannii potential of hydrazine derivatives. Considering this scenario, we aimed to evaluate the antimicrobial and antibiofilm activity of 10 pyridinylhydrazone compounds against A. baumannii . The minimum inhibitory concentration of the compounds was determined by broth microdilution method and the antibiofilm activity was evaluated by inhibition and destruction biofilm assays. In addition, the cytotoxicity of the compounds in the J774A.1 cell line was also evaluated, and the selectivity index was calculated. Among the 10 pyridine compounds, the compounds B and D were able to inhibit the formation of biofilms and destroy bacterial biofilms even in a concentration of 12.5 μg/mL. Thus, the pyridine compounds evaluated can be a scaffold for the development of new substances with antimicrobial and antibiofilm activity. |
