Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism.

Autor: Houston BJ; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia.; School of Biological Sciences, Faculty of Science, Monash University, Clayton, VIC, 3800, Australia., Nixon B; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia.; Hunter Medical Research Institute, Cancer Research Program, New Lambton Heights, NSW, 2305, Australia., McEwan KE; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia., Martin JH; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia.; Hunter Medical Research Institute, Cancer Research Program, New Lambton Heights, NSW, 2305, Australia., King BV; School of Mathematical and Physical Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia., Aitken RJ; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia.; Hunter Medical Research Institute, Cancer Research Program, New Lambton Heights, NSW, 2305, Australia., De Iuliis GN; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia. geoffry.deiuliis@newcastle.edu.au.; Hunter Medical Research Institute, Cancer Research Program, New Lambton Heights, NSW, 2305, Australia. geoffry.deiuliis@newcastle.edu.au.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Nov 25; Vol. 9 (1), pp. 17478. Date of Electronic Publication: 2019 Nov 25.
DOI: 10.1038/s41598-019-53983-9
Abstrakt: Artificially generated radiofrequency-electromagnetic energy (RF-EME) is now ubiquitous in our environment owing to the utilization of mobile phone and Wi-Fi based communication devices. While several studies have revealed that RF-EME is capable of eliciting biological stress, particularly in the context of the male reproductive system, the mechanistic basis of this biophysical interaction remains largely unresolved. To extend these studies, here we exposed unrestrained male mice to RF-EME generated via a dedicated waveguide (905 MHz, 2.2 W/kg) for 12 h per day for a period of 1, 3 or 5 weeks. The testes of exposed mice exhibited no evidence of gross histological change or elevated stress, irrespective of the RF-EME exposure regimen. By contrast, 5 weeks of RF-EME exposure adversely impacted the vitality and motility profiles of mature epididymal spermatozoa. These spermatozoa also experienced increased mitochondrial generation of reactive oxygen species after 1 week of exposure, with elevated DNA oxidation and fragmentation across all exposure periods. Notwithstanding these lesions, RF-EME exposure did not impair the fertilization competence of spermatozoa nor their ability to support early embryonic development. This study supports the utility of male germ cells as sensitive tools with which to assess the biological impacts of whole-body RF-EME exposure.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje