Optimized Genetic Testing for Polledness in Multiple Breeds of Cattle.

Autor: Randhawa IAS; School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia, i.randhawa@uq.edu.au., Burns BM; Department of Agriculture and Fisheries, Rockhampton, QLD 4702, Australia., McGowan MR; School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia., Porto-Neto LR; CSIRO Agriculture and Food, St Lucia, QLD 4067, Australia., Hayes BJ; Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St Lucia, QLD 4072, Australia., Ferretti R; Bioinformatics and Biostatistics, Neogen GeneSeek, Lincoln, NE, 68504 and., Schutt KM; Neogen Australasia, University of Queensland, Gatton, QLD 4343, Australia., Lyons RE; School of Veterinary Science, University of Queensland, Gatton, QLD 4343, Australia.; Neogen Australasia, University of Queensland, Gatton, QLD 4343, Australia.
Jazyk: angličtina
Zdroj: G3 (Bethesda, Md.) [G3 (Bethesda)] 2020 Feb 06; Vol. 10 (2), pp. 539-544. Date of Electronic Publication: 2020 Feb 06.
DOI: 10.1534/g3.119.400866
Abstrakt: Many breeds of modern cattle are naturally horned, and for sound husbandry management reasons the calves frequently undergo procedures to physically remove the horns by disbudding or dehorning. These procedures are however a welfare concern. Selective breeding for polledness - absence of horns - has been effective in some cattle breeds but not in others ( Bos indicus genotypes) due in part to the complex genetics of horn phenotype. To address this problem different approaches to genetic testing which provide accurate early-in-life prediction of horn phenotype have been evaluated, initially using microsatellites (MSAT) and more recently single nucleotide polymorphism (SNP). A direct gene test is not effective given the genetic heterogeneity and large-sized sequence variants associated with polledness in different breeds. The current study investigated 39,943 animals of multiple breeds to assess the accuracy of available poll testing assays. While the standard SNP-based test was an improvement on the earlier MSAT haplotyping method, 1999 (9.69%) out of 20,636 animals tested with this SNP-based assay did not predict a genotype, most commonly associated with the Indicus-influenced breeds. The current study has developed an optimized poll gene test that resolved the vast majority of these 1999 unresolved animals, while the predicted genotypes of those previously resolved remained unchanged. Hence the optimized poll test successfully predicted a genotype in 99.96% of samples assessed. We demonstrated that a robust set of 5 SNPs can effectively determine P C and P F alleles and eliminate the ambiguous and undetermined results of poll gene testing previously identified as an issue in cattle.
(Copyright © 2020 Randhawa et al.)
Databáze: MEDLINE
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