| Autor: |
Abril D; Bacterial Molecular Genetics Laboratory, Universidad El Bosque, Carrera 9 Nº131A-02, Bogota D.C. 110121, Colombia., Bustos Moya IG; Grupo de Medicina Cardiovascular y especialidades de alta complejidad-Fundación Clínica Shaio, Bogota D.C. 110121, Colombia., Marquez-Ortiz RA; Bacterial Molecular Genetics Laboratory, Universidad El Bosque, Carrera 9 Nº131A-02, Bogota D.C. 110121, Colombia., Josa Montero DF; Grupo de Medicina Cardiovascular y especialidades de alta complejidad-Fundación Clínica Shaio, Bogota D.C. 110121, Colombia., Corredor Rozo ZL; Bacterial Molecular Genetics Laboratory, Universidad El Bosque, Carrera 9 Nº131A-02, Bogota D.C. 110121, Colombia., Torres Molina I; Grupo de Medicina Cardiovascular y especialidades de alta complejidad-Fundación Clínica Shaio, Bogota D.C. 110121, Colombia., Vanegas Gómez N; Bacterial Molecular Genetics Laboratory, Universidad El Bosque, Carrera 9 Nº131A-02, Bogota D.C. 110121, Colombia.; The i3 institute, Faculty of Science University of Technology, Sydney PO Box 123, Australia., Escobar-Perez J; Bacterial Molecular Genetics Laboratory, Universidad El Bosque, Carrera 9 Nº131A-02, Bogota D.C. 110121, Colombia. |
| Abstrakt: |
The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the bla OXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other bla OXA-244 -harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The bla OXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn 6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a bla OXA-244 -harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America. |
